상세 보기
- Chen, Zexiang;
- Kwan, Suet-Yan;
- Mir, Aamir;
- Hazeltine, Max;
- Shin, Minwook;
- 외 11명
WEB OF SCIENCE
6SCOPUS
5초록
CRISPR-based genome-editing technologies, including nuclease editing, base editing, and prime editing, have recently revolutionized the development of therapeutics targeting disease-causing mutations. To advance the assessment and development of genome editing tools, a robust mouse model is valuable, particularly for evaluating in vivo activity and delivery strategies. In this study, we successfully generated a knock-in mouse line carrying the Traffic Light Reporter design known as TLR-multi-Cas variant 1 (TLR-MCV1). We comprehensively validated the functionality of this mouse model for both in vitro and in vivo nuclease and prime editing. The TLR-MCV1 reporter mouse represents a versatile and powerful tool for expediting the development of editing technologies and their therapeutic applications.
키워드
- 제목
- A Fluorescent Reporter Mouse for In Vivo Assessment of Genome Editing with Diverse Cas Nucleases and Prime Editors
- 저자
- Chen, Zexiang; Kwan, Suet-Yan; Mir, Aamir; Hazeltine, Max; Shin, Minwook; Liang, Shun-Qing; Chan, Io Long; Kelly, Karen; Ghanta, Krishna S.; Gaston, Nicholas; Cao, Yueying; Xie, Jun; Gao, Guangping; Xue, Wen; Sontheimer, Erik J.; Watts, Jonathan K.
- 발행일
- 2023-12
- 유형
- Article
- 저널명
- CRISPR JOURNAL
- 권
- 6
- 호
- 6
- 페이지
- 570 ~ 582