Immobilized Candida antarctica lipase B as an sn-1,3 regiospecific biocatalyst for the interesterification of triacylglycerols with fatty acid ethyl esters
Immobilized Candida antarctica lipase B as an sn-1,3 regiospecific biocatalyst for the interesterification of triacylglycerols with fatty acid ethyl esters
  • Hwang, Jihyun
  • Aum, Jiae
  • Lee, Seong Jae
  • Mun, Jeong Min
  • Kim, Seung Wook
  • ... Kim, Byung Hee
  • 외 2명
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초록

Candida antarctica lipase B (CALB) is regarded as non-regiospecific. This study aimed to investigate the regiospecificity of CALB in the solvent-free interesterification of high-oleic sunflower oil with stearic acid ethyl ester for 1,3-distearoyl-2-oleoylglycerol (SOS)-rich fat preparation using a packed bed reactor. The content ratio of 1,2-distearoyl-3-oleoylglycerol (SSO) to SOS (denoted by SSO/SOS content) obtained using Lipozyme 435 (a commercially immobilized CALB; 0–4.1%), at residence times (1–32 min) was similar to that obtained using Lipozyme RM IM (0–3.0%), but lower than that obtained using Lipozyme TL IM (6.0–39.4%). When immobilized on Lewatit VP OC 1600, Lipozyme CALB had an SSO/SOS content of 0–10.4%, which was greater than that of Palatase 20,000 L (0–1.1%) but was lower than that of Lipozyme TL 100 L (8.8–97.7%). Our findings suggest that immobilized CALB shows distinct sn-1,3 regiospecificity in the interesterification of triacylglycerol with fatty acid ethyl esters.

키워드

Candida antarctica Lipase BImmobilizationInteresterificationLipozyme 435Sn-1,3 specificityCOCOA BUTTER EQUIVALENTSENZYMATIC INTERESTERIFICATIONACYL MIGRATIONPALM STEARINSOYBEAN OILSESAME OILLIQUIDTRANSESTERIFICATIONHYDROLYSISSOLVENT
제목
Immobilized Candida antarctica lipase B as an sn-1,3 regiospecific biocatalyst for the interesterification of triacylglycerols with fatty acid ethyl esters
제목 (타언어)
Immobilized Candida antarctica lipase B as an sn-1,3 regiospecific biocatalyst for the interesterification of triacylglycerols with fatty acid ethyl esters
저자
Hwang, JihyunAum, JiaeLee, Seong JaeMun, Jeong MinKim, Seung WookChung, Min‑YuKim, In‑HwanKim, Byung Hee
DOI
10.1007/s10068-023-01337-7
발행일
2024-01
유형
Article
저널명
Food Science and Biotechnology
33
1
페이지
159 ~ 170