Proteomic analysis of time-dependant difference of protein expression profile changes during neuronal differentiation of mouse embryonic stem cells
  • Shim, Jung Hee
  • Cho, Sun A.
  • Seo, Min Ji
  • Kim, Jung Hee
  • Ryu, Na Kyung
  • ... Yoo, Kyung Hyun
  • 외 4명
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초록

The study of ES cell-mediated neuronal differentiation allows elucidating the mechanism of neuronal development in spite of the complexity and the difficult accessibility. During the differentiation of embryonic stem cells into neuronal cell, the expression profiles in the level of protein were extensively investigated by proteomic analysis. These cells were analyzed for charges in proteome during the differentiation of ES cells by 2-dimensional electrophoresis (2-DE) and MALDI-TOF MS. Seven unique proteins were identified, some of which were differentially expressed at each stage. A complex system of neuronal differentiation can be activated in cultured embryonic stem cells and our two dimensional electrophoresis data should be useful for investigating some of the mechanism that regulates neuronal differentiation.

키워드

2-DEDifferentiationES cellNeuronalProteomic analysisIN-VITROHUMAN BLASTOCYSTSLINESDISEASEMODEL
제목
Proteomic analysis of time-dependant difference of protein expression profile changes during neuronal differentiation of mouse embryonic stem cells
저자
Shim, Jung HeeCho, Sun A.Seo, Min JiKim, Jung HeeRyu, Na KyungYoo, Kyung HyunYang, Moon HeeKim, SeyoonBahk, Young YilPark, Jong Hoon
DOI
10.1007/s10059-010-0022-2
발행일
2010-03
유형
Article
저널명
Molecules and Cells
29
3
페이지
239 ~ 244