상세 보기
- Park, Mi Soon;
- Wee, Chido;
- Lee, Junsoo;
- Kim, Byung Hee;
- Kim, Hak-Ryul;
- 외 1명
WEB OF SCIENCE
3SCOPUS
3초록
The docosahexaenoic acid (DHA) was concentrated from tuna oil fatty acid using solvent crystallization combined with lipase-catalyzed ethanolysis. In the first step, solvent crystallization was carried out to concentrate DHA from tuna oil fatty acid using acetonitrile as a solvent. The optimal conditions of solvent crystallization were the crystallization temperature of -40 degrees C and the fatty acid to solvent ratio of 1:8 (w/v). This step increased the DHA content in the original tuna oil fatty acid from 22% up to 61%. In the second step, lipase-catalyzed ethanolysis was conducted with DHA-enriched fatty acid from the first step using Lipozyme RM IM (from Rhizomucor miehei) as a biocatalyst. The optimum conditions of this second step were the reaction temperature of 20 degrees C and the molar ratio of 1:1 (fatty acid to ethanol). Overall, DHA enrichment with purity of 85% was obtained by the two step processes.
키워드
- 제목
- Concentration of docosahexsaenoic acid from tuna oil by a combination of solvent crystallization and lipase-catalyzed ethanolysis
- 저자
- Park, Mi Soon; Wee, Chido; Lee, Junsoo; Kim, Byung Hee; Kim, Hak-Ryul; Kim, In-Hwan
- 발행일
- 2024-07
- 유형
- Article; Early Access
- 권
- 101
- 호
- 7
- 페이지
- 637 ~ 645