ScholarWorks@숙명여자대학교

초록

Ethnopharmacological relevance: Chaga mushroom (Inonotus obliquus,10) has been used as a folk remedy for cancer, digestive system diseases, and other illnesses in Russia and Eastern Europe. Aim of the study: In the present study, we investigated the immunomodulating effects of 10 through in vivo and ex vivo studies. Materials and methods: Serum immunoglobulins (1gE, IgG(1), and IgG(2a)) and cytokines (interleukin (1L)-4, interferon (1FN)-gamma, and IL-2) were measured in concanavalin A (ConA)-stimulated splenocytes and CD4(+) T cells. The nitric oxide (NO) secretion of lipopolysaccharide (LPS)-stimulated peritoneal macrophages was also measured after oral administration of 50, 100, or 200 mg kg(-1) d(-1) IO hot water extract (IOE) to ovalbumin (OVA)-sensitized BALB/c mice. Results: We found that the OVA-induced increase in serum IgE and IgG(2a) was significantly suppressed when IOE was orally administered after the second immunization with OVA. ConA stimulation in spleen cells isolated from OVA-sensitized mice treated with 100 mg kg IOE resulted in a 25.2% decrease in IL-4 production and a 102.4% increase in IFN-gamma, compared to the controls. Moreover, IL-4, IFN-gamma, and IL-2 were significantly reduced after ConA stimulation in isolated CD4(+)T cells. We also determined that IOE inhibits the secretion of NO from LPS-stimulated peritoneal macrophages ex vivo. Conclusions: We suggest that IO modulates immune responses through secretion of Th1/Th2 cytokines in immune cells and regulates antigen-specific antibody production. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

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