A colorimetric hydrogel biosensor for rapid detection of nitrite ions
  • Nam, Jihye
  • Jung, In-Bok
  • Kim, Boyoon
  • Lee, Sang-Myung
  • Kim, Seong-Eun
  • ... Shin, Dong-Sik
  • 외 1명
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110
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120

초록

Nitrite ions are important markers of inflammation, food safety, and water quality; therefore, various methods are under development to detect nitrite ions. This paper describes a novel method for detecting nitrite ions using polyethylene glycol (PEG) hydrogel superimposed glass fiber membrane strips. A colorimetric Griess assay was performed on the hydrogel modified with N-(1-naphthyl)ethylenediamine by dropping a solution of nitrite and sulfanilamide. This colorimetric hydrogel sensor exhibited a detection limit as low as 10 mu M for nitrite ions and the working concentration of nitrite ions was between 10 mu M and 5 mM. The colorimetric reaction was completed within a few seconds; the performance test results indicated that the sensor was specific to nitrite ions compared to other anions, and it was suitable to detect nitrite ions in clinical samples. In the future, this sensor platform can be used to detect nitrite ions in various complex solutions including biological and environmental samples. (C) 2018 Elsevier B.V. All rights reserved.

키워드

Nitrite ionsRapid detectionGriess assayGlass fiberHydrogelCARBON-PASTE ELECTRODESTRIP BIOSENSORACETYLCHOLINESTERASE BIOSENSORSENSITIVE DETECTIONULCERATIVE-COLITISGRIESS REACTIONOXIDE SYNTHASENITRATECELLSNANOPARTICLES
제목
A colorimetric hydrogel biosensor for rapid detection of nitrite ions
저자
Nam, JihyeJung, In-BokKim, BoyoonLee, Sang-MyungKim, Seong-EunLee, Kook-NyungShin, Dong-Sik
DOI
10.1016/j.snb.2018.04.171
발행일
2018-10
유형
Article
저널명
Sensors and Actuators, B: Chemical
270
페이지
112 ~ 118