Carabrol suppresses LPS-induced nitric oxide synthase expression by inactivation of p38 and JNK via inhibition of I-kappa B alpha degradation in RAW 264.7 cells
  • Lee, Hwa Jin
  • Lim, Hyo Jin
  • Lee, Da Yeon
  • Jung, Hyeyoun
  • Kim, Mi-Ran
  • ... Kim, Keun Il
  • 외 3명
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초록

Carabrol, isolated from Carpesium macrocephalum, showed anti-inflammatory potential in LPS-induced RAW 264.7 murine macrophages. In present study, carabrol demonstrated the inhibitory activity on pro-inflammatory cytokines such as IL-1 beta, IL-6 and TNF-alpha. In addition, mRNA and protein levels of iNOS and COX-2 were reduced by carabrol. Molecular analysis revealed that these suppressive effects were correlated with the inactivation of p38 and JNK via inhibition of NF-kappa B activation. Immunoblotting showed that carabrol suppressed LPS-induced degradation of I-kappa B alpha and decreased nuclear translocation of p65. Taken together, these results suggest that carabrol can be a modulator of pro-inflammatory signal transduction pathway in RAW 264.7 cells. (C) 2009 Elsevier Inc. All rights reserved.

키워드

CarabrolNitric oxideProstaglandin E-2Carpesium macrocephalumNuclear factor-kappa BACTIVATED PROTEIN-KINASEMACROPHAGESLIPOPOLYSACCHARIDEINFLAMMATIONPATHWAYINOSERKCYCLOOXYGENASEBIOSYNTHESISINDUCTION
제목
Carabrol suppresses LPS-induced nitric oxide synthase expression by inactivation of p38 and JNK via inhibition of I-kappa B alpha degradation in RAW 264.7 cells
저자
Lee, Hwa JinLim, Hyo JinLee, Da YeonJung, HyeyounKim, Mi-RanMoon, Dong-CheulKim, Keun IlLee, Myeong-SokRyu, Jae-Ha
DOI
10.1016/j.bbrc.2009.12.073
발행일
2010-01
유형
Article
저널명
Biochemical and Biophysical Research Communications
391
3
페이지
1400 ~ 1404