상세 보기
- Kim, Se-Ri;
- Yoon, Yohan;
- Seo, Min-Kyoung;
- Kim, Won-Il;
- Shim, Won-Bo;
- 외 4명
WEB OF SCIENCE
3SCOPUS
4초록
To establish the best detection method for Escherichia coli O157:H7 on produce, the sensitivity, specificity, and recovery values of 3 selective media [sorbitol MacConkey agar containing cefixime and tellurite (CTSMAC), sorbitol MacConkey agar containing cefixime, tellurite, and 5-bromo-4-chloro-3-indoxyl-beta-glucuronide (CTB-SMAC), and CHROMagar O157 were compared. Sample preparation methods (pummeling, sonication, and hand-shaking) were compared to determine the optimum procedure for qualitative analysis from cherry tomato. The selectivity and recovery rates of stressed cell of the 3 tested media were in the order of CHROMagar O157 > CTBSMAC > CT-SMAC. The recovery rates of hand-shaken tomatoes (10/10) were higher than rates of sonicated (8/10) and pummeled tomatoes (6/10) for inoculated cherry tomatoes in modified E. coli (mEC) broth enriched with novobicin. CHROMagar O157 was the optimum agar medium for detection of E. coli O157:H7. Cherry tomatoes should be treated with hand-shaking to enhance recovery for qualitative analysis.
키워드
- 제목
- Modification of methods for detection of Escherichia coli O157:H7 on produce
- 저자
- Kim, Se-Ri; Yoon, Yohan; Seo, Min-Kyoung; Kim, Won-Il; Shim, Won-Bo; Chung, Duck Hwa; Yun, Jong Chul; Ryu, Kyoung Yul; Kim, Byung-Seok
- 발행일
- 2014-08
- 유형
- Article
- 권
- 23
- 호
- 4
- 페이지
- 1349 ~ 1356