ScholarWorks@숙명여자대학교

초록

The objective of this study is to evaluate the anticancer effects of the isoflavone extract from Chungkukjangin human breast cancer, MDA-MB-453 cells. For this study, MDA-MB-453 cells were treated with 12.5, 25, and 50 μg isoflavoneextract for 24, 48, and 72 hr. Cell proliferations were decreased in a time- and dose-dependent manner. Reduced cellproliferation was suspected by apoptosis or cell cycle arrest. Therefore, after treatment of 50 μg isoflavone extract, apoptoticcells were investigated by annexin V staining. The results indicated that isoflavone extract increased the number of earlyapoptotic cells compared with control. Cleaved PARP was also increased. Next, we investigated the cell cycle and relatedproteins. The isoflavone extract leads to cell cycle arrest at the G2/M phase. Moreover isoflavone extract had influencedcell cycle relate proteins such as cyclin B1, cyclin A, and p21. These results suggest that isoflavone extract from Chungkukjanginduce apoptosis and cell cycle arrest at G2/M phase via regulation of cell cycle-related proteins in MDA-MB-453cells.

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