Cloning and sequence analyses of a 2,3-dihydroxybiphenyl 1,2-dioxygenase gene (bphC) from Comamonas sp. SMN4 for phylogenetic and structural analysis

Lee, NR; Kwon, DY; Min, KH

doi:10.1007/s10295-003-0039-z

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Cloning and sequence analyses of a 2,3-dihydroxybiphenyl 1,2-dioxygenase gene (bphC) from Comamonas sp. SMN4 for phylogenetic and structural analysis

Lee, NR;
Kwon, DY;
Min, KH

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4

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SCOPUS

5

초록

A genomic library of biphenyl-degrading Comamonas sp. SMN4 for isolating fragments containing the 2,3-dihydroxybiphenyl 1,2-dioxygenase (23DBDO) gene was constructed. The smallest subclone (pNPX9) encoding 23DBDO activity was sequenced and analyzed. The C-terminal domain of 23DBDO from Comamonas sp. SMN4 had five catalytically essential residues and was more highly conserved than the N-terminal domain. Phylogenetic and structural relationships of 23DBDO from Comamonas sp. SMN4 were analyzed.

키워드

dihydroxybiphenyl dioxygenase; bphC; extradiol dioxygenase; Comamonas; biphenyl degradation; SP STRAIN KKS102; EXTRADIOL DIOXYGENASE; NUCLEOTIDE-SEQUENCE; PSEUDOMONAS-PUTIDA; BIPHENYL; DEGRADATION; CLEAVAGE; ENZYME

제목: Cloning and sequence analyses of a 2,3-dihydroxybiphenyl 1,2-dioxygenase gene (bphC) from Comamonas sp. SMN4 for phylogenetic and structural analysis

저자: Lee, NR; Kwon, DY; Min, KH

DOI: 10.1007/s10295-003-0039-z

발행일: 2003-04

유형: Article

저널명: Journal of Industrial Microbiology and Biotechnology

권: 30

호: 4

페이지: 245 ~ 250