상세 보기
초록
Polycystic kidney disease (PKD) is one of the most common inherited disorders, involving progressive cyst formation in the kidney that leads to renal failure. FK506 binding protein 12 (FK506BP) is an immunophilin protein that performs multiple functions, including regulation of cell signaling pathways and survival. In this study, we determined the roles of PEP-1-FK506BP on cell proliferation and cyst formation in PKD cells. Purified PEP-1-FK506BP transduced into PKD cells markedly inhibited cell proliferation. Also, PEP-1-FK506BP drastically inhibited the expression levels of p-Akt, p-p70S6K, p-mTOR, and p-ERK in PKD cells. In a 3D-culture system, PEP-1-FK506BP significantly reduced cyst formation. Furthermore, the combined effects of rapamycin and PEP-1-FK506BP on cyst formation were markedly higher than the effects of individual treatments. These results suggest that PEP-1-FK506BP delayed cyst formation and could be a new therapeutic strategy for renal cyst formation in PKD.
키워드
- 제목
- Effects of PEP-1-FK506BP on cyst formation in polycystic kidney disease
- 저자
- Jo, Hyo Sang; Eum, Won Sik; Park, Eun Young; Ko, Je Young; Kim, Do Yeon; Kim, Dae Won; Shin, Min Jea; Son, Ora; Cho, Su Bin; Park, Jung Hwan; Lee, Chi Hern; Yeo, Eun Ji; Yeo, Hyeon Ji; Choi, Yeon Joo; Youn, Jong Kyu; Cho, Sung-Woo; Park, Jinseu; Park, Jong Hoon; Choi, Soo Young
- 발행일
- 2017-09
- 유형
- Article
- 저널명
- BMB Reports
- 권
- 50
- 호
- 9
- 페이지
- 460 ~ 465