Inhibitory effect of a sesquiterpene from artemisia iwayomogi on expression of inducible nitric oxide synthase by suppression of I-κBα degradation in LPS-stimulated RAW 264.7 cells
  • Kim, Na Yeon
  • Koh, Hye Jin
  • Li, Hua
  • Lee, Hwa Jin
  • Ryu, Jae-Ha
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초록

A sesquiterpene was purified from Artemisia iwayomogi methanolic extract during the course of searching anti-inflammatory principle from medicinal plants. A sesquiterpene identified as armefolin inhibited the production of nitric oxide (NO) and attenuated inducible nitric oxide synthase (iNOS) protein level in lipopoly-saccharide (LPS)-activated RAW 264.7 cells. Armefolin also down-regulated mRNA expressions of iNOS and pro-inflammatory cytokines, interleukin-1β and interleukin-6 in LPS-activated macrophages. Moreover, armefolin suppressed the degradation of inhibitory-κBα (I-κBα) in LPS-activated macrophages. These data suggest that armefolin from A. iwayomogi can suppress the LPS-induced production of NO and the expression of iNOS gene through inhibiting the degradation of I-κBα. Taken together, armefolin from A. iwayomogi might be a candidate as promising anti-inflammatory agent. © 2017, Korean Society of Pharmacognosy. All rights reserved.

키워드

ArmefolinArtemisia iwayomogiInducible nitric oxide synthaseNitric oxideSesquiterpeneI kappa B kinase alphainducible nitric oxide synthaseinterleukin 1betainterleukin 6lipopolysaccharidemessenger RNAnitric oxidesesquiterpeneanimal cellArtemisiaArtemisia iwayomogiArticlecell viabilitycontrolled studydown regulationdrug mechanismdrug structuregene expression regulationIL 1beta geneIL 6 geneiNOS genemacrophage activationnonhumanprotein degradationprotein expressionproton nuclear magnetic resonanceRAW 264.7 cell linereverse transcription polymerase chain reactionupregulation
제목
Inhibitory effect of a sesquiterpene from artemisia iwayomogi on expression of inducible nitric oxide synthase by suppression of I-κBα degradation in LPS-stimulated RAW 264.7 cells
저자
Kim, Na YeonKoh, Hye JinLi, HuaLee, Hwa JinRyu, Jae-Ha
DOI
10.20307/nps.2017.23.2.92
발행일
2017-06
유형
Article
저널명
Natural Product Sciences
23
2
페이지
92 ~ 96