상세 보기
- Kim, Jiwon;
- Chung, Min-Yu;
- Choi, Hee-Don;
- Choi, In-Wook;
- Kim, Byung Hee
WEB OF SCIENCE
20SCOPUS
20초록
We enzymatically prepared structured monogalactosydiacylglycerols (MGDGs) enriched in pinolenic acid (PLA). PLA-enriched free fatty acids (FFAs) containing similar to 86 mol % PLA were produced from an FFA fraction obtained from pine nut oil (PLA content, similar to 13 mol %) by urea crystallization. Commercial MGDGs (5 mg) were acidolyzed with PLA-enriched FFAs using four commercial immobilized lipases as biocatalysts. The reaction was performed in acetone (4 mL) in a stirred-batch reactor. Lipozyme RM IM (immobilized Rhizomucor miehei lipase) was the most effective biocatalyst for the reaction. Structured MGDGs containing 42.1 mol % PLA were obtained under optimal reaction conditions: temperature, 25 degrees C; substrate molar ratio, 1:30 (MGDGs/PLA-enriched FFAs); enzyme loading, 20 wt % of total substrates; and reaction time, 36 h. The structured MGDGs were separated from the reaction products at a purity of 96.6 wt % using silica column chromatography. The structured MGDGs could be possibly used as emulsifiers with appetite-suppression effects.
키워드
- 제목
- Enzymatic Synthesis of Structured Monogalactosyldiacylglycerols Enriched in Pinolenic Acid
- 저자
- Kim, Jiwon; Chung, Min-Yu; Choi, Hee-Don; Choi, In-Wook; Kim, Byung Hee
- 발행일
- 2018-08
- 유형
- Article
- 권
- 66
- 호
- 30
- 페이지
- 8079 ~ 8085