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초록
We developed trimethyl lock quinone-functionalized coumarin (probe 1) as a small fluorescent probe for the imaging of NQO1 activity in the live cells. NQO1 is highly expressed in most cancer cells and it is considered as a potential biomarker of NQO1-related cancer. Probe 1 showed the high selectivity and rapid fluorescence response to the NQO1 activity. In addition, the probe is biocompatible and easily penetrates living cells. It was found that probe 1 provided relatively strong fluorescence in NQO1-positive A549 cells, unlike NQO1-negative NIH-3T3 and MDA-MB-231 cells. Therefore, we demonstrated that probe 1 can detect NQO1 activity in real time in living cells and it can also distinguish different levels of NQO1 activity in various pathological cells.
키워드
- 제목
- Trimethyl Lock Quinone-Functionalized Coumarin for Real-time Monitoring of NQO1 Activity in the Live Cells
- 저자
- Park, Sun Young; Yoon, Shin A; Lee, Min Hee
- 발행일
- 2021-01
- 권
- 42
- 호
- 1
- 페이지
- 119 ~ 123