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초록
We have presented rhodamine-derived Schiff base probes (1-6) selective of the Fe3+ ion in biological systems. In the presence of Fe3+ ions, the probes display new absorption and emission bands at 526 nm and 551 nm, respectively. Confocal microscopic analysis using the HepG2 cell line showed that probes 1-6 provide fluorescence Off-On changes caused by cellular Fe3+ ions, and demonstrated a largely enhanced fluorescence upon iron overloading of the cells. We also found that the Fe3+ ion-mediated fluorescence changes of probes 1-6 are mainly localized in the ER (Endoplasmic Reticulum) in HepG2 cells. Among them, probe 5 exhibits the highest selectivity for ER localization over the other organelles. (C) 2016 Elsevier Ltd. All rights reserved.
키워드
Iron detection; Fluorescent probe; Rhodamine; Cell imaging; MOLECULAR CONTROL; IRON-METABOLISM
- 제목
- A fluorescent probe for the Fe3+ ion pool in endoplasmic reticulum in liver cells
- 저자
- Lee, Min Hee; Lee, Hoyeon; Chang, Min Jung; Kim, Hyeong Seok; Kang, Chulhun; Kim, Jong Seung
- 발행일
- 2016-07
- 유형
- Article
- 권
- 130
- 페이지
- 245 ~ 250