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초록
We examined the ways in which fenobam could promote not only the transduction of PEP-1-FK506BP into cells and tissues but also the neuroprotective effect of PEP-1-FK506BP against ischemic damage. Fenobam strongly enhanced the protective effect of PEP-1-FK506BP against H2O2-induced toxicity and DNA fragmentation in C6 cells. In addition, combinational treatment of fenobam with PEP-1-FK506BP significantly inhibited the activation of Akt and MAPK induced by H2O2, compared to treatment with PEP-1-FK506BP alone. Interestingly, our results showed that fenobam significantly increased the transduction of PEP-1-FK506BP into both C6 cells and the hippocampus of gerbil brains. Subsequently, a transient ischemic gerbil model study demonstrated that fenobam pretreatment led to the increased neuroprotection of PEP-1-FK506BP in the CA1 region of the hippocampus. Therefore, these results suggest that fenobam can be a useful agent to enhance the transduction of therapeutic PEP-1-fusion proteins into cells and tissues, thereby promoting their neuroprotective effects.
키워드
- 제목
- Fenobam promoted the neuroprotective effect of PEP-1-FK506BP following oxidative stress by increasing its transduction efficiency
- 저자
- Ahn, Eun Hee; Kim, Dae Won; Shin, Min Jea; Jo, Hyo Sang; Eom, Seon Ae; Kim, Duk-Soo; Park, Eun Young; Park, Jong Hoon; Cho, Sung-Woo; Park, Jinseu; Eum, Won Sik; Son, Ora; Hwang, Hyun Sook; Choi, Soo Young
- 발행일
- 2013-11
- 유형
- Article
- 저널명
- BMB Reports
- 권
- 46
- 호
- 11
- 페이지
- 561 ~ 566