상세 보기
- Song, S. -H.;
- Kim, B. -M.;
- Lim, C. -J.;
- Song, Y. S.;
- Park, E. -H.
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1초록
This work was designed to assess regulation of the atf1+ gene in the fission yeast Schizosaccharomyces pombe under nitrosative and nutritional stresses, using the atf1+-lacZ fusion gene and RT-PCR. Nitric oxide (NO)-generating sodium nitroprusside (SNP; 10 mu mol/L) and nitrogen depletion significantly enhanced synthesis of beta-galactosidase from the atf1+-lacZ fusion gene in S. pombe Pap1-positive KP1 cells, but not in S. pombe Pap1-negative TP108-3C cells. SNP (10 mu mol/L) and nitrogen depletion also caused a significant increase in atf1+ mRNA levels in Pap1-positive cells, but not in Pap1-negative cells. Depletion of glucose marginally increased synthesis of beta-galactosidase from the fusion gene in S. pombe Pap1-positive cells. Taken together, the S. pombe atf1+ gene is upregulated by nitrosative and nutritional stresses on a transcriptional level, possibly via the mediation of Pap1.
키워드
- 제목
- Expression of the atf1(+) gene is upregulated in fission yeast under nitrosative and nutritional stresses
- 저자
- Song, S. -H.; Kim, B. -M.; Lim, C. -J.; Song, Y. S.; Park, E. -H.
- DOI
- 10.1139/W09-087
- 발행일
- 2009-11
- 유형
- Article
- 권
- 55
- 호
- 11
- 페이지
- 1323 ~ 1327