Peroxisome proliferator-activated receptor beta/delta (PPAR beta/delta) activates promyogenic signaling pathways, thereby promoting myoblast differentiation
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초록

Peroxisome proliferator-activated receptor NS (PPAR beta/delta) regulates postnatal myogenesis by alleviating myostatin activity, but the molecular mechanisms by which it regulates myogenesis are not fully understood. In this study, we investigate molecular mechanisms of PPAR beta/delta in myoblast differentiation. C2C12 myoblasts treated with a PPAR beta/delta agonist, GW0742 exhibit enhanced myotube formation and muscle-specific gene expression. GW0742 treatment dramatically activates promyogenic kinases, p38MAPK and Akt, in a dose-dependent manner. GW0742-stimulated myoblast differentiation is mediated by p38MAPK and Akt, since it failed to restore myoblast differentiation repressed by inhibition of p38MAPK and Akt. In addition, GW0742 treatment enhances MyoD-reporter activities. Consistently, overexpression of PPAR beta/delta enhances myoblast differentiation accompanied by elevated activation of p38MAPK and Akt. Collectively, these results suggest that PPAR beta/delta enhances myoblast differentiation through activation of promyogenic signaling pathways. (C) 2016 Elsevier Inc. All rights reserved.

키워드

AktMyoblast differentiationp38MAPKPromyogenic signalingPPAR beta/deltaMYOGENIC DIFFERENTIATIONSKELETAL-MUSCLEPROTEIN-KINASECDODELTAMETABOLISMCOMPLEXOBESITY
제목
Peroxisome proliferator-activated receptor beta/delta (PPAR beta/delta) activates promyogenic signaling pathways, thereby promoting myoblast differentiation
저자
Lee, Sang-JinGo, Ga-YeonYoo, MiranKim, Yong KeeSeo, Dong-WanKang, Jong-SunBae, Gyu-Un
DOI
10.1016/j.bbrc.2016.01.012
발행일
2016-01
유형
Article
저널명
Biochemical and Biophysical Research Communications
470
1
페이지
157 ~ 162