Clusterin, a novel modulator of TGF-beta signaling, is involved in Smad2/3 stability
  • Lee, Kwan-Bok
  • Jeon, Jun-Ho
  • Choi, Inpyo
  • Kwon, O-Yu
  • Yu, Kweon
  • 외 1명
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초록

Clusterin (CLU) is known as a multifunctional protein involved in a variety of physiological processes including lipid transport, epithelial cell differentiation, tumorigenesis, and apoptosis. It is known that CLU interacts with TGF-beta type 11 receptor (T beta R11). However, the relationship of CLU and TGF-beta signaling is unclear. Here we present that CLU is a novel modulator of TGF-beta signaling by regulating Smad2/3 proteins. Overexpression of CLU enhanced TGF-beta-induced transcriptional activity and increased the amount of Smad2/3 proteins, while CLU siRNA repressed TGF-beta-induced transcriptional activity and decreased the amount of Smad2/3 proteins in Hep3B cells. We also found that CLU was involved in Smad2/3 stability at the protein level. These findings suggest that CLU regulates TGF-beta signaling pathway by modulating the stability of Smad2/3 proteins. (c) 2007 Elsevier Inc. All rights reserved.

키워드

clusterinTGF-betaSmad2Smad3I-KAPPA-BUBIQUITIN-DEPENDENT DEGRADATIONPROTEINSCHAPERONETRANSCRIPTIONHOMOLOGCOMPLEXSTRESSALPHADEATH
제목
Clusterin, a novel modulator of TGF-beta signaling, is involved in Smad2/3 stability
저자
Lee, Kwan-BokJeon, Jun-HoChoi, InpyoKwon, O-YuYu, KweonYou, Kwan-Hee
DOI
10.1016/j.bbrc.2007.12.033
발행일
2008-02-22
유형
Article
저널명
Biochemical and Biophysical Research Communications
366
4
페이지
905 ~ 909