PDE4 inhibitor upregulates PTH-induced osteoclast formation via CRE-mediated COX-2 expression in osteoblasts
  • Park, Hyojung
  • No, A. Long Sae Mi
  • Lee, Jung-Min
  • Chen, Ling
  • Lee, Soo Young
  • ... Yim, Mijung
  • 외 1명
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초록

We investigated the interplay between parathyroid hormone (PTH) and phosphodiesterases (PDEs) in osteoblasts. PDE4 negatively regulated PTH-induced cAMP accumulation. PDE4 inhibitor enhanced PTH-induced osteoclast formation and RANKL mRNA expression, which is partially mediated by COX-2 mRNA expression. Two CRE sites in the COX-2 promoter were required for the increase in COX-2 transcription by PDE4 inhibitor, and the expression of a dominant-negative form of CREB abolished COX-2 mRNA expression in response to PDE4 inhibitor or PTH in osteoblasts. Taken together, our data indicate that PDE4 inhibitor promotes PTH-induced osteoclast formation partially via CRE-mediated COX-2 mRNA expression. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

키워드

Parathyroid hormonePhosphodiesterase 4RANKLCOX-2CREOsteoblastOsteoclastNECROSIS-FACTOR RECEPTORLIGANDDIFFERENTIATIONKINASECELLSPROTEINTRANCE/RANKLINVOLVEMENTBIOLOGY
제목
PDE4 inhibitor upregulates PTH-induced osteoclast formation via CRE-mediated COX-2 expression in osteoblasts
저자
Park, HyojungNo, A. Long Sae MiLee, Jung-MinChen, LingLee, Soo YoungLee, Dong-SeokYim, Mijung
DOI
10.1016/j.febslet.2009.11.043
발행일
2010-01-04
유형
Article
저널명
FEBS Letters
584
1
페이지
173 ~ 180