상세 보기
- Miyamoto, Arei;
- Takami, Masamichi;
- Matsumoto, Akifumi;
- Mochizuki, Ayako;
- Yamada, Takako;
- ... Yim, Mijung;
- 외 8명
WEB OF SCIENCE
13SCOPUS
14초록
R848, also known as resiquimod, acts as a ligand for toll-like receptor 7 (TLR7) and activates immune cells. In this study, we examined the effects of R848 on differentiation, survival, and bone-resorbing function of osteoclasts. R848 inhibited osteoclast differentiation of mouse bone marrow-derived macrophages (BMMs) and human peripheral blood-derived monocytes induced by receptor activator of NF-kappa B ligand in a dose-dependent manner. In addition, it inhibited mouse osteoclast differentiation induced in cocultures of bone marrow cells and osteoblasts in the presence of dihydroxyvitamin D-3 [1,25(OH)(2)D-3]. However, R848 did not affect the survival or bone-resorbing activity of mouse mature osteoclasts. R848 also upregulated the mRNA expression levels of interleukin (IL)-6, IL-12, interferon (IFN)-gamma, and inducible nitric oxide synthase in mouse BMMs expressing TLR7. IFN-beta was consistently expressed in the BMMs and addition of neutralizing antibodies against IFN-beta to the cultures partially recovered osteoclast differentiation inhibited by R848. These results suggest that R848 targets osteoclast precursors and inhibits their differentiation into osteoclasts via TLR7.
키워드
- 제목
- R848, a toll-like receptor 7 agonist, inhibits osteoclast differentiation but not survival or bone-resorbing function of mature osteoclasts
- 저자
- Miyamoto, Arei; Takami, Masamichi; Matsumoto, Akifumi; Mochizuki, Ayako; Yamada, Takako; Tachi, Keita; Shibuya, Isao; Nakamachi, Tomoya; Shioda, Seiji; Baba, Kazuyoshi; Inoue, Tomio; Miyamoto, Yoichi; Yim, Mijung; Kamijo, Ryutaro
- 발행일
- 2012-05
- 유형
- Article; Proceedings Paper
- 저널명
- Cytotechnology
- 권
- 64
- 호
- 3
- 페이지
- 331 ~ 339