낙엽송층버섯으로부터 Ergosterol 유도체의 분리 및 RAW 264.7 세포주의 Nitric Oxide 생성 저해활성
Inhibition of Nitric Oxide Synthesis by Ergosterol Derivative from Phellinus pini in LPS-ActivatedRAW 264.7 Cells
  • 장현진
  • 양기숙
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초록

Phellinus pini (Hymenocaetaceae) has ben used for the immunomodulating activity, hypolipidemic effect, gas-tric cancer, non-insulin dependant diabetes, diarhea, and menstrual irregularity. From the screening of each fraction for theinhibitory activity of NO production in lipopolysacaride (LPS) activated RAW 264.7 cells, methanol extract of Phellinus piniand hexane soluble fraction exhibited inhibition of NO production compared with LPS control without toxicity. The hexanehexane fr. was repeatedly chromatographed over silica gel, ergosta-4,6,8(14),22- tetraen-3-one was isolated. The compoundinhibited NOS activation .IC50 : 29.7 uM) and NO production of activated macrophage at 30 uM.Keywords . Phelinus pini, ergosta-4,6,8(14),22-tetraen-3-one, nitric oxide, RAW 264.7 cells...... Phellinus pini(Brot ex Fr.) Ames. .....(Basidiomycotina), ...... (Aphyllophorales), ........ (Hymenochaetaceae)1). ... , ... .. ..... ,... , ... .. .... .... .... .... ... (... ).... .... .. . ... white rot fungus. .... ... ..... lignin. .... ... ... ... ..... , ....... .. .... .. .

키워드

Phellinus piniergosta-468(14)22-tetraen-3-onenitric oxideRAW 264.7 cells
제목
낙엽송층버섯으로부터 Ergosterol 유도체의 분리 및 RAW 264.7 세포주의 Nitric Oxide 생성 저해활성
제목 (타언어)
Inhibition of Nitric Oxide Synthesis by Ergosterol Derivative from Phellinus pini in LPS-ActivatedRAW 264.7 Cells
저자
장현진양기숙
발행일
2006-12
저널명
약 학 회 지
50
6
페이지
367 ~ 371