Selective Estrogen Receptor Modulation by Larrea nitida on MCF-7 Cell Proliferation and Immature Rat Uterus
DC Field | Value | Language |
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dc.contributor.author | Ahn, Hye-Na | - |
dc.contributor.author | Jeong, Si-Yeon | - |
dc.contributor.author | Bae, Gyu-Un | - |
dc.contributor.author | Chang, Minsun | - |
dc.contributor.author | Zhang, Dongwei | - |
dc.contributor.author | Liu, Xiyuan | - |
dc.contributor.author | Pei, Yihua | - |
dc.contributor.author | Chin, Young-Won | - |
dc.contributor.author | Lee, Joongku | - |
dc.contributor.author | Oh, Sei-Ryang | - |
dc.contributor.author | Song, Yun Seon | - |
dc.date.available | 2021-02-22T11:48:22Z | - |
dc.date.issued | 2014-07 | - |
dc.identifier.issn | 1976-9148 | - |
dc.identifier.issn | 2005-4483 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/10868 | - |
dc.description.abstract | Larrea nitida is a plant that belongs to the Zygophyllaceae family and is widely used in South America to treat inflammatory diseases, tumors and menstrual pain. However, its pharmacological activity remains unclear. In this study we evaluated the property of selective estrogen receptor modulator (SERM) of Larrea nitida extracts (LNE) as a phytoestrogen that can mimic, modulate or disrupt the actions of endogenous estrogens, depending on the tissue and relative amount of other SERMs. To investigate the property of SERM of LNE, we performed MCF-7 cell proliferation assays, estrogen response element (ERE)-luciferase reporter gene assay, human estrogen receptor (hER) binding assays and in vivo uterotrophic assay. To gain insight into the active principles, we performed a bioassay-guided analysis of LNE employing solvents of various polarities and using classical column chromatography, which yielded 16 fractions (LNs). LNE showed high binding affinities for hER alpha and hER beta with 10(50) values of 1.20 x 10(-7) g/ml and 1.00 x 10(-7) g/ml, respectively. LNE induced 17 beta-estradiol (E2)-induced MCF-7 cell proliferation, however, it reduced the proliferation in the presence of E2. Furthermore, LNE had an atrophic effect in the uterus of immature rats through reducing the expression level of progesterone receptor (PR) proteins. LN08 and LN10 had more potent affinities for binding on hER alpha and beta than other fractions. Our results indicate that LNE had higher binding affinities for hER beta than hER alpha, and showed SERM properties in MCF-7 breast cancer cells and the rat uterus. LNE may be useful for the treatment of estrogen-related conditions, such as female cancers and menopause. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC APPLIED PHARMACOLOGY | - |
dc.title | Selective Estrogen Receptor Modulation by Larrea nitida on MCF-7 Cell Proliferation and Immature Rat Uterus | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.4062/biomolther.2014.050 | - |
dc.identifier.scopusid | 2-s2.0-84905216693 | - |
dc.identifier.wosid | 000340020600013 | - |
dc.identifier.bibliographicCitation | BIOMOLECULES & THERAPEUTICS, v.22, no.4, pp 347 - 354 | - |
dc.citation.title | BIOMOLECULES & THERAPEUTICS | - |
dc.citation.volume | 22 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 347 | - |
dc.citation.endPage | 354 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001898327 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | HUMAN BREAST-CANCER | - |
dc.subject.keywordPlus | HUMAN PROGESTERONE-RECEPTOR | - |
dc.subject.keywordPlus | B-ISOFORM | - |
dc.subject.keywordPlus | NORDIHYDROGUAIARETIC ACID | - |
dc.subject.keywordPlus | TISSUE-SPECIFICITY | - |
dc.subject.keywordPlus | TUMOR-CELLS | - |
dc.subject.keywordPlus | A-ISOFORM | - |
dc.subject.keywordPlus | BETA | - |
dc.subject.keywordPlus | TRIDENTATA | - |
dc.subject.keywordPlus | LIGNANS | - |
dc.subject.keywordAuthor | Larrea nitida | - |
dc.subject.keywordAuthor | Lignan | - |
dc.subject.keywordAuthor | Phytoestrogen | - |
dc.subject.keywordAuthor | Estrogen receptor | - |
dc.subject.keywordAuthor | MCF-7 cell | - |
dc.subject.keywordAuthor | Uterus | - |
dc.identifier.url | http://www.biomolther.org/journal/view.html?volume=22&number=4&spage=347&year=2014 | - |
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