NDRG2 overexpression enhances glucose deprivation-mediated apoptosis in breast cancer cells via inhibition of the LKB1-AMPK pathway
DC Field | Value | Language |
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dc.contributor.author | Kim H.-S. | - |
dc.contributor.author | Kim M.-J. | - |
dc.contributor.author | Lim J. | - |
dc.contributor.author | Yang Y. | - |
dc.contributor.author | Lee M.-S. | - |
dc.contributor.author | Lim J.-S. | - |
dc.date.available | 2021-02-22T11:48:40Z | - |
dc.date.issued | 2014-05 | - |
dc.identifier.issn | 1947-6019 | - |
dc.identifier.issn | 1947-6027 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/10899 | - |
dc.description.abstract | The newly identified tumor suppressor, N-myc downstream-regulated gene 2 (NDRG2), has been studied in various cancers because of its anticancer and antimetastasis effects. In this study, we examined the effect of NDRG2 expression on cell viability in MDA-MB-231 human breast cancer cells under conditions that are similar to the microenvironment of solid tumors, which include glucose deprivation. NDRG2 overexpression enhanced the pro-apoptotic effects of glucose deprivation. Glucose deprivation also induced the activation of AMP-activated protein kinase (AMPK), which plays a role in protecting tumor cells from metabolic stresses. NDRG2 overexpression strongly reduced glucose deprivation-induced AMPK phosphorylation and increased the cleavage of poly (ADP-ribose) polymerase (PARP), which indicated the induction of apoptosis. The expression of a constitutively active form of AMPK effectively blocked glucose deprivation-induced apoptosis in NDRG2-overexpressing MDA-MB-231 cells. Moreover, NDRG2 overexpression also enhanced the pro-apoptoti ceffects of 2-deoxyglucose (2-DG) or hypoxia, an inducer of metabolic stresses. Finally, we showed that LKB1 is an upstream kinase of AMPK that is involved in the inhibition of glucose deprivation-induced AMPK activity in NDRG2-overexpressing cells. Our findings collectively suggest that NDRG2 is a negative regulator of AMPK activity and functions as a sensitizer of glucose deprivation. © 2014, Impact Journals LLC. All rights reserved. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Impact Journals LLC | - |
dc.title | NDRG2 overexpression enhances glucose deprivation-mediated apoptosis in breast cancer cells via inhibition of the LKB1-AMPK pathway | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.18632%2Fgenesandcancer.17 | - |
dc.identifier.scopusid | 2-s2.0-84928382725 | - |
dc.identifier.bibliographicCitation | Genes and Cancer, v.5, no.5-6, pp 175 - 185 | - |
dc.citation.title | Genes and Cancer | - |
dc.citation.volume | 5 | - |
dc.citation.number | 5-6 | - |
dc.citation.startPage | 175 | - |
dc.citation.endPage | 185 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | deoxyglucose | - |
dc.subject.keywordPlus | hydroxymethylglutaryl coenzyme A reductase kinase | - |
dc.subject.keywordPlus | protein kinase LKB1 | - |
dc.subject.keywordPlus | apoptosis | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | breast cancer | - |
dc.subject.keywordPlus | cancer inhibition | - |
dc.subject.keywordPlus | cell death | - |
dc.subject.keywordPlus | cell viability | - |
dc.subject.keywordPlus | colon cancer | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | cytotoxicity | - |
dc.subject.keywordPlus | flow cytometry | - |
dc.subject.keywordPlus | fluorescence activated cell sorting | - |
dc.subject.keywordPlus | genetic transfection | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | hypoxia | - |
dc.subject.keywordPlus | metabolic stress | - |
dc.subject.keywordPlus | plasmid | - |
dc.subject.keywordPlus | priority journal | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | reverse transcription polymerase chain reaction | - |
dc.subject.keywordPlus | RNA extraction | - |
dc.subject.keywordPlus | signal transduction | - |
dc.subject.keywordPlus | tumor microenvironment | - |
dc.subject.keywordPlus | Western blotting | - |
dc.subject.keywordAuthor | AMPK | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Glucose deprivation | - |
dc.subject.keywordAuthor | NDRG2 | - |
dc.identifier.url | https://www.genesandcancer.com/article/17/text/ | - |
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