PEP-1-PON1 Protein Regulates Inflammatory Response in Raw 264.7 Macrophages and Ameliorates Inflammation in a TPA-Induced Animal Model
DC Field | Value | Language |
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dc.contributor.author | Kim, Mi Jin | - |
dc.contributor.author | Jeong, Hoon Jae | - |
dc.contributor.author | Kim, Dae Won | - |
dc.contributor.author | Sohn, Eun Jeong | - |
dc.contributor.author | Jo, Hyo Sang | - |
dc.contributor.author | Kim, Duk-Soo | - |
dc.contributor.author | Kim, Hyun Ah | - |
dc.contributor.author | Park, Eun Young | - |
dc.contributor.author | Park, Jong Hoon | - |
dc.contributor.author | Son, Ora | - |
dc.contributor.author | Han, Kyu Hyung | - |
dc.contributor.author | Park, Jinseu | - |
dc.contributor.author | Eum, Won Sik | - |
dc.contributor.author | Choi, Soo Young | - |
dc.date.available | 2021-02-22T12:01:43Z | - |
dc.date.issued | 2014-01 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/11025 | - |
dc.description.abstract | Paraoxonase 1 (PON1) is an antioxidant enzyme which plays a central role in various diseases. However, the mechanism and function of PON1 protein in inflammation are poorly understood. Since PON1 protein alone cannot be delivered into cells, we generated a cell permeable PEP-1-PON1 protein using protein transduction domains, and examined whether it can protect against cell death in lipopolysaccharide (LPS) or hydrogen peroxide (H2O2)-treated Raw 264.7 cells as well as mice with 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced skin inflammation. We demonstrated that PEP-1-PON1 protein transduced into Raw 264.7 cells and markedly protected against LPS or H2O2-induced cell death by inhibiting cellular reactive oxygen species (ROS) levels, the inflammatory mediator's expression, activation of mitogen-activated protein kinases (MAPKs) and cellular apoptosis. Furthermore, topically applied PEP-1-PON1 protein ameliorates TPA-treated mice skin inflammation via a reduction of inflammatory response. Our results indicate that PEP-1-PON1 protein plays a key role in inflammation and oxidative stress in vitro and in vivo. Therefore, we suggest that PEP-1-PON1 protein may provide a potential protein therapy against oxidative stress and inflammation. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | PEP-1-PON1 Protein Regulates Inflammatory Response in Raw 264.7 Macrophages and Ameliorates Inflammation in a TPA-Induced Animal Model | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1371/journal.pone.0086034 | - |
dc.identifier.scopusid | 2-s2.0-84899812894 | - |
dc.identifier.wosid | 000330288000041 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.9, no.1 | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 9 | - |
dc.citation.number | 1 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | MURINE ALVEOLAR MACROPHAGES | - |
dc.subject.keywordPlus | NEURONAL CELL-DEATH | - |
dc.subject.keywordPlus | DISEASE MOUSE MODEL | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | PARKINSONS-DISEASE | - |
dc.subject.keywordPlus | COX-2 EXPRESSION | - |
dc.subject.keywordPlus | ANTIINFLAMMATORY ACTIVITIES | - |
dc.subject.keywordPlus | SERUM PARAOXONASE | - |
dc.subject.keywordPlus | MICE | - |
dc.identifier.url | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0086034 | - |
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