Self-Calibrating Bipartite Fluorescent Sensor for Nitroreductase Activity and Its Application to Cancer and Hypoxic Cells
DC Field | Value | Language |
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dc.contributor.author | Yoon, Shin A | - |
dc.contributor.author | Chun, Jieun | - |
dc.contributor.author | Kang, Chulhun | - |
dc.contributor.author | Lee, Min Hee | - |
dc.date.available | 2021-02-22T05:21:42Z | - |
dc.date.issued | 2021-03 | - |
dc.identifier.issn | 2576-6422 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/1136 | - |
dc.description.abstract | Aromatic nitro compounds are reduced to their corresponding amino derivatives by nitroreductases (NTR), while identification and characterization of the corresponding enzymes in mammalian systems are yet unrevealed. However, mammalian NTR activity has been considered as a favorable target in development of theranostic agents for cancer and hypoxia of solid tumors. Currently, small molecule-based fluorescent probes have emerged as a valuable assay tool for NTR activity. However, there has been a limit to comparing NTR activity between different cells, since most probes have relied on fluorescence changes that are affected by not only enzymatic activity but also nonenzymatic factors. Here, we developed a self-calibrating bipartite fluorescent probe, consisting of NTR-sensitive nitronaphthalimide and nonsensitive coumarin moieties. Thereby, it was possible to compare the relative NTR activity by monitoring fluorescence ratios in noncancerous and some cancerous cells and to demonstrate for certain that the elevated NTR activity is associated with cancer cells and hypoxia states. © XXXX American Chemical Society. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Chemical Society | - |
dc.title | Self-Calibrating Bipartite Fluorescent Sensor for Nitroreductase Activity and Its Application to Cancer and Hypoxic Cells | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1021/acsabm.0c01085 | - |
dc.identifier.scopusid | 2-s2.0-85094568581 | - |
dc.identifier.wosid | 000630168200008 | - |
dc.identifier.bibliographicCitation | ACS Applied Bio Materials, v.4, no.3, pp 2052 - 2057 | - |
dc.citation.title | ACS Applied Bio Materials | - |
dc.citation.volume | 4 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 2052 | - |
dc.citation.endPage | 2057 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | esci | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | Cells | - |
dc.subject.keywordPlus | Cytology | - |
dc.subject.keywordPlus | Diseases | - |
dc.subject.keywordPlus | Mammals | - |
dc.subject.keywordPlus | Probes | - |
dc.subject.keywordPlus | Theranostics | - |
dc.subject.keywordPlus | Aromatic nitro compounds | - |
dc.subject.keywordPlus | Enzymatic activities | - |
dc.subject.keywordPlus | Favorable targets | - |
dc.subject.keywordPlus | Fluorescence ratio | - |
dc.subject.keywordPlus | Fluorescent probes | - |
dc.subject.keywordPlus | Fluorescent sensors | - |
dc.subject.keywordPlus | Nitroreductase activity | - |
dc.subject.keywordPlus | Theranostic agents | - |
dc.subject.keywordPlus | Fluorescence | - |
dc.subject.keywordAuthor | bioimaging | - |
dc.subject.keywordAuthor | bipartite fluorescent probe | - |
dc.subject.keywordAuthor | cancer cells | - |
dc.subject.keywordAuthor | hypoxia | - |
dc.subject.keywordAuthor | nitroreductase | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acsabm.0c01085 | - |
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