A Gene Trap Knockout of the Tiam-1 Protein Results in Malformation of the Early Embryonic Brain
DC Field | Value | Language |
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dc.contributor.author | Yoo, Sooyeon | - |
dc.contributor.author | Kim, Yujin | - |
dc.contributor.author | Lee, Haeryung | - |
dc.contributor.author | Park, Sungjeong | - |
dc.contributor.author | Park, Soochul | - |
dc.date.available | 2021-02-22T12:46:09Z | - |
dc.date.issued | 2012-07 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.issn | 0219-1032 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/11876 | - |
dc.description.abstract | Tiam-1 has been implicated in the development of the central nervous system. However, the in vivo function of Tiam-1 has not been fully determined in the developing mouse brain. In this study, we generated Tiam-1 knockout mice using a Tiam-1 gene-trapped embryonic stem cell line. Insertion of a gene trap vector into a genomic site downstream of exon 5 resulted in a mutant allele encoding a truncated protein fused with the beta-geo LacZ gene. Primary mouse embryonic fibroblasts lacking Tiam-1 revealed a significant decrease in Rac activity and cell proliferation. In addition, whole-mount embryonic LacZ expression analysis demonstrated that Tiam-1 is specifically expressed in regions of the developing brain, such as the caudal telencephalon and rostral diencephalon. More importantly, mouse embryos deficient in Tiam-1 gene expression displayed a severe defect in embryonic brain development, including neural tube closure defects or a dramatic decrease in brain size. These findings suggest that embryonic Tiam-1 expression plays a critical role during early brain development in mice. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.title | A Gene Trap Knockout of the Tiam-1 Protein Results in Malformation of the Early Embryonic Brain | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.1007/s10059-012-0119-x | - |
dc.identifier.scopusid | 2-s2.0-84864465548 | - |
dc.identifier.wosid | 000307273600013 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.34, no.1, pp 103 - 108 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 34 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 103 | - |
dc.citation.endPage | 108 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001682492 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | EXCHANGE FACTOR TIAM1 | - |
dc.subject.keywordPlus | INVASION | - |
dc.subject.keywordPlus | RAC | - |
dc.subject.keywordPlus | POLARITY | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | STEF/TIAM1 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordAuthor | early brain development | - |
dc.subject.keywordAuthor | Rac | - |
dc.subject.keywordAuthor | Tiam-1 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs10059-012-0119-x | - |
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