Over-expression of Mxi1 represses renal epithelial tubulogenesis through the reduction of matrix metalloproteinase 9
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, Seon Ah | - |
dc.contributor.author | Yoo, Kyung Hyun | - |
dc.contributor.author | Ko, Je Yeong | - |
dc.contributor.author | Kim, Bo Hye | - |
dc.contributor.author | Yook, Yeon Joo | - |
dc.contributor.author | Park, Jong Hoon | - |
dc.date.available | 2021-02-22T12:47:01Z | - |
dc.date.issued | 2012-03 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/11960 | - |
dc.description.abstract | Autosomal dominant polycystic kidney disease (ADPKD) is the most common hereditary renal disease. ADPKD is characterized by cyst development that leads to abnormal kidney structure. Renal tubules are a fundamental unit of architecture, so controls of tubular growth and formation are important for proper kidney function. The molecular mechanisms of tubulogenesis are being actively studied as the basis of diagnosis and treatment of ADPKD. Mxi1 is a member of the MAD family of proteins that functions in terminal differentiation, inhibition of cell cycle progression and tumor suppression, while the Myc protein, which is antagonized by Mxi1, causes renal cystogenesis. Based on these molecular relationships, the present study implicated Mxi1 with ADPKD be demonstrating that curtailed Mxi1 gene expression caused cyst formation in Mxi1-deficient mice. To ascertain whether Mxi1 affects renal epithelial cell tubulogenesis, three-dimensional cultures (3D culture) of mIMCD-3 cells and stably Mxi1 over-expressed mIMCD-3 cells were established. The results indicated that over-expression of the Mxi1 gene plays a role in the regulation of tubulogenesis by regulating some genes participating in renal epithelial branching tubulogenesis such as matrix metalloproteinase 9 (MMP9), integrins, fibronectin, and E-cadherin. The results support the suggestion that over-expression of Mxi1 can suppress renal epithelial tubulogenesis. In particular, MMP9 is greatly affected by the expression level of Mxi1. It can be concluded that mIMCD-3 cells that stably over-express Mxi1 fail to form renal epithelial tubules because of abnormally reduced expression of MMP9. (C) 2012 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Over-expression of Mxi1 represses renal epithelial tubulogenesis through the reduction of matrix metalloproteinase 9 | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.bbrc.2012.02.002 | - |
dc.identifier.scopusid | 2-s2.0-84862803728 | - |
dc.identifier.wosid | 000302437100002 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.419, no.3, pp 459 - 465 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 419 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 459 | - |
dc.citation.endPage | 465 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | POLYCYSTIC KIDNEY-DISEASE | - |
dc.subject.keywordPlus | BRANCHING MORPHOGENESIS | - |
dc.subject.keywordPlus | URETERAL BUD | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | FIBRONECTIN | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | MYC | - |
dc.subject.keywordPlus | MAX | - |
dc.subject.keywordAuthor | Mxi1 | - |
dc.subject.keywordAuthor | MMP9 | - |
dc.subject.keywordAuthor | Tubulogenesis | - |
dc.subject.keywordAuthor | mIMCD-3 cells | - |
dc.subject.keywordAuthor | Three-dimensional culture | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/abs/pii/S0006291X12002203?via%3Dihub | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Sookmyung Women's University. Cheongpa-ro 47-gil 100 (Cheongpa-dong 2ga), Yongsan-gu, Seoul, 04310, Korea02-710-9127
Copyright©Sookmyung Women's University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.