PLC/PRF/5 인체 간암 세포에서 Luteolin에 의한 TRAIL 감수성 증가 효과Luteolin-induced Sensitization of PLC/PRF/5 Human Hepatocellular Carcinoma Cells to TRAIL-Mediated Apoptosis
- Other Titles
- Luteolin-induced Sensitization of PLC/PRF/5 Human Hepatocellular Carcinoma Cells to TRAIL-Mediated Apoptosis
- Authors
- 김은영; 김안근
- Issue Date
- Mar-2012
- Publisher
- 대한암예방학회
- Keywords
- TRAIL; PLC/PRF/5; Luteolin; Combination; Apoptosis
- Citation
- 대한암예방학회지, v.17, no.1, pp 37 - 44
- Pages
- 8
- Journal Title
- 대한암예방학회지
- Volume
- 17
- Number
- 1
- Start Page
- 37
- End Page
- 44
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/12296
- ISSN
- 2288-3649
2288-3657
- Abstract
- Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a member of the tumor necrosis factor super family, has been expected as a promising anticancer agent because of its ability to induce apoptosis in malignant cells but not in normal cells. However, some cancer cells have resistance mechanisms to TRAIL-mediated apoptosis. Strategies to enhance potential of TRAIL are therefore needed for effective TRAIL therapy. In this study, we showed that luteolin enhances TRAIL-induced cytotoxicity in PLC/PRF/5 human hepatocellular carcinoma cells, whereas acacetin, caffeic acid phenethyl ester, quercetin produces no effect. Synergistic induction of apoptosis was confirmed by flow cytometric analysis after annexin V/PI double staining. Also, PARP-cleavage and activation of effector caspases were significantly enhanced by TRAIL/luteolin combination compared to either agent alone. Furthermore, activation of Bid and Bax/Bcl-2 ratio up-regulation were markedly enhanced as well as caspase-8 and caspase-9 activation by TRAIL/luteolin combined treatment, suggesting that both extrinsic and intrinsic apoptotic pathway are triggered in PLC/PRF/5 cells treated with TRAIL and luteolin combination.
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