Activation of the interleukin-32 pro-inflammatory pathway in response to human papillomavirus infection and over-expressionof interleukin-32 controls the expression of the humanpapillomavirus oncogene
DC Field | Value | Language |
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dc.contributor.author | Lee, Sojung | - |
dc.contributor.author | Kim, Jung-Hee | - |
dc.contributor.author | Kim, Heejong | - |
dc.contributor.author | Kang, Jeong Woo | - |
dc.contributor.author | Kim, Soo-Hyun | - |
dc.contributor.author | Yang, Young | - |
dc.contributor.author | Kim, Jinman | - |
dc.contributor.author | Park, JongSup | - |
dc.contributor.author | Park, SurNie | - |
dc.contributor.author | Hong, JinTae | - |
dc.contributor.author | Yoon, Do-Young | - |
dc.date.available | 2021-02-22T13:18:04Z | - |
dc.date.issued | 2011-03 | - |
dc.identifier.issn | 0019-2805 | - |
dc.identifier.issn | 1365-2567 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/12633 | - |
dc.description.abstract | P>High-risk variants of human papillomavirus (HPV) induce cervical cancer by persistent infection, and are regarded as the principal aetiological factor in this malignancy. The pro-inflammatory cytokine interleukin-32 (IL-32) is present at substantial levels in cervical cancer tissues and in HPV-positive cervical cancer cells. In this study, we identified the mechanism by which the high-risk HPV-16 E7 oncogene induces IL-32 expression in cervical cancer cells. We used antisense transfection, over-expression, or knock-down of IL-32 to assess the effects of the HPV-16 E7 oncogene on IL-32 expression in cervical cancer cells. Cyclo-oxygenase 2 (COX-2) inhibitor treatment was conducted, and the expression levels, as well as the promoter activities, of IL-32 and COX-2 were evaluated in human HPV-positive cervical cancer cell lines. E7 antisense treatment reduced the expression levels and promoter activities of COX-2, which is constitutively expressed in HPV-infected cells. Constitutively expressed IL-32 was also inhibited by E7 antisense treatment. Moreover, IL-32 expression was blocked by the application of the selective COX-2 inhibitor, NS398, whereas COX-2 over-expression resulted in increased IL-32 levels. These results show that the high-risk variant of HPV induces IL-32 expression via E7-mediated COX-2 stimulation. However, E7 and COX-2 were down-regulated in the IL-32 gamma over-expressing cells and recovered by IL-32 small interfering RNA, indicating that E7 and COX-2 were feedback-inhibited by IL-32 gamma in cervical cancer cells. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY | - |
dc.title | Activation of the interleukin-32 pro-inflammatory pathway in response to human papillomavirus infection and over-expressionof interleukin-32 controls the expression of the humanpapillomavirus oncogene | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1111/j.1365-2567.2010.03377.x | - |
dc.identifier.scopusid | 2-s2.0-79251627826 | - |
dc.identifier.wosid | 000286839300011 | - |
dc.identifier.bibliographicCitation | IMMUNOLOGY, v.132, no.3, pp 410 - 420 | - |
dc.citation.title | IMMUNOLOGY | - |
dc.citation.volume | 132 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 410 | - |
dc.citation.endPage | 420 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | CERVICAL-CANCER | - |
dc.subject.keywordPlus | E7 ONCOPROTEINS | - |
dc.subject.keywordPlus | MESSENGER-RNAS | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | HPV16 E7 | - |
dc.subject.keywordPlus | IL-32 | - |
dc.subject.keywordPlus | E6 | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | cervical cancer | - |
dc.subject.keywordAuthor | cyclo-oxygenase-2 | - |
dc.subject.keywordAuthor | feedback inhibition | - |
dc.subject.keywordAuthor | human papillomavirus E7 | - |
dc.subject.keywordAuthor | interleukin-32 | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2567.2010.03377.x | - |
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