Activation-Tagged Suppressors of a Weak Brassinosteroid Receptor Mutant
DC Field | Value | Language |
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dc.contributor.author | Kang, Bin | - |
dc.contributor.author | Wang, Hao | - |
dc.contributor.author | Nam, Kyoung Hee | - |
dc.contributor.author | Li, Jiayang | - |
dc.contributor.author | Li, Jianming | - |
dc.date.available | 2021-02-22T13:49:04Z | - |
dc.date.issued | 2010-01 | - |
dc.identifier.issn | 1674-2052 | - |
dc.identifier.issn | 1752-9867 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/13296 | - |
dc.description.abstract | Brassinosteroids (BRs) are important plant hormones that act synergistically with auxin to regulate a variety of plant developmental and physiological processes. In the past decade, genetic and biochemical studies have revealed a linear signaling pathway that relies on protein phosphorylation to transmit the BR signal into the nucleus, altering expression of hundreds of genes to promote plant growth. We conducted an activation-tagging based suppressor screen to look for Arabidopsis genes that, when overexpressed by inserted 35S enhancer elements, could suppress the dwarf phenotype of a weak BR receptor mutant bri1-301. This screen identified a total of six dominant activation-tagged bri1 suppressors (atbs-Ds). Using a plasmid rescue approach, we discovered that the bri1-301 suppression effect in four atbs-D mutants (atbs3-D to atbs6-D) was caused by overexpression of a YUCCA gene thought to be involved in tryptophan-dependent auxin biosynthesis. Interestingly, the three activation-tagged YUCCA genes belong to the YUCCA IIA subfamily that includes two other members out of 11 known Arabidopsis YUCCA genes. In addition, our molecular studies revealed a T-DNA insertion near a basic helix-loop-helix gene in atbs1-D and a T-DNA insertion in a region carrying a BR biosynthetic gene in atbs2-D. Further studies of these atbs-D mutants could lead to better understanding of the BR signaling process and the BR-auxin interaction. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | CELL PRESS | - |
dc.title | Activation-Tagged Suppressors of a Weak Brassinosteroid Receptor Mutant | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1093/mp/ssp099 | - |
dc.identifier.scopusid | 2-s2.0-76549127749 | - |
dc.identifier.wosid | 000273893200019 | - |
dc.identifier.bibliographicCitation | MOLECULAR PLANT, v.3, no.1, pp 260 - 268 | - |
dc.citation.title | MOLECULAR PLANT | - |
dc.citation.volume | 3 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 260 | - |
dc.citation.endPage | 268 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.subject.keywordPlus | ARABIDOPSIS-THALIANA | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTORS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | FLAVIN MONOOXYGENASES | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM | - |
dc.subject.keywordPlus | AUXIN BIOSYNTHESIS | - |
dc.subject.keywordPlus | PROTEIN-KINASE | - |
dc.subject.keywordPlus | BRI1 | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordAuthor | Brassinosteroid | - |
dc.subject.keywordAuthor | BRI1 | - |
dc.subject.keywordAuthor | bri1-301 | - |
dc.subject.keywordAuthor | auxin | - |
dc.subject.keywordAuthor | YUCCA | - |
dc.identifier.url | https://www.cell.com/molecular-plant/fulltext/S1674-2052(14)60411-1?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1674205214604111%3Fshowall%3Dtrue | - |
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