Expression of the atf1(+) gene is upregulated in fission yeast under nitrosative and nutritional stresses
DC Field | Value | Language |
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dc.contributor.author | Song, S. -H. | - |
dc.contributor.author | Kim, B. -M. | - |
dc.contributor.author | Lim, C. -J. | - |
dc.contributor.author | Song, Y. S. | - |
dc.contributor.author | Park, E. -H. | - |
dc.date.available | 2021-02-22T14:16:05Z | - |
dc.date.issued | 2009-11 | - |
dc.identifier.issn | 0008-4166 | - |
dc.identifier.issn | 1480-3275 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/13658 | - |
dc.description.abstract | This work was designed to assess regulation of the atf1+ gene in the fission yeast Schizosaccharomyces pombe under nitrosative and nutritional stresses, using the atf1+-lacZ fusion gene and RT-PCR. Nitric oxide (NO)-generating sodium nitroprusside (SNP; 10 mu mol/L) and nitrogen depletion significantly enhanced synthesis of beta-galactosidase from the atf1+-lacZ fusion gene in S. pombe Pap1-positive KP1 cells, but not in S. pombe Pap1-negative TP108-3C cells. SNP (10 mu mol/L) and nitrogen depletion also caused a significant increase in atf1+ mRNA levels in Pap1-positive cells, but not in Pap1-negative cells. Depletion of glucose marginally increased synthesis of beta-galactosidase from the fusion gene in S. pombe Pap1-positive cells. Taken together, the S. pombe atf1+ gene is upregulated by nitrosative and nutritional stresses on a transcriptional level, possibly via the mediation of Pap1. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS | - |
dc.title | Expression of the atf1(+) gene is upregulated in fission yeast under nitrosative and nutritional stresses | - |
dc.type | Article | - |
dc.publisher.location | 캐나다 | - |
dc.identifier.doi | 10.1139/W09-087 | - |
dc.identifier.scopusid | 2-s2.0-73449085173 | - |
dc.identifier.wosid | 000273418700012 | - |
dc.identifier.bibliographicCitation | CANADIAN JOURNAL OF MICROBIOLOGY, v.55, no.11, pp 1323 - 1327 | - |
dc.citation.title | CANADIAN JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 55 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1323 | - |
dc.citation.endPage | 1327 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | SCHIZOSACCHAROMYCES-POMBE | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | LACZ FUSIONS | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | PHASE | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordAuthor | Atf1 | - |
dc.subject.keywordAuthor | nitric oxide | - |
dc.subject.keywordAuthor | nitrogen starvation | - |
dc.subject.keywordAuthor | Schizosaccharomyces pombe | - |
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