The neuronal differentiation potential of Ldb1-null mutant embryonic stem cells is dependent on extrinsic influences
DC Field | Value | Language |
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dc.contributor.author | Hwang, M. | - |
dc.contributor.author | Gorivodsky, M. | - |
dc.contributor.author | Kim, Min Jung | - |
dc.contributor.author | Westphal, H. | - |
dc.contributor.author | Geum, D. | - |
dc.date.available | 2021-02-22T14:45:55Z | - |
dc.date.issued | 2008-06 | - |
dc.identifier.issn | 1066-5099 | - |
dc.identifier.issn | 1549-4918 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/14244 | - |
dc.description.abstract | LIM-domain binding protein 1 (Ldb1) is a multiadaptor protein that mediates the action of transcription factors, including LIM-homeodomain proteins. To elucidate the functional role of Ldb1 in the neuronal differentiation of embryonic stem (ES) cells, we have generated Ldb1-null mutant (Ldb1-/-) ES cells and examined neuronal differentiation potentials in vitro using two different neuronal differentiation protocols. When subjected to a five-stage protocol that recapitulates in vivo conditions of neuronal differentiation, wild-type ES cells differentiated into a wide spectrum of neuronal cell types. However, Ldb1-/- ES cells did not differentiate into neuronal cells; instead, they differentiated into sarcomeric α-actinin-positive muscle cells. In contrast, when an adherent monolayer culture procedure (which is based on the default mechanism of neural induction and eliminates environmental influences) was applied, both wild-type and Ldb1-/- ES cells differentiated into MAP2-positive mature neurons. Comparison of the results obtained when two different neuronal differentiation protocols were used suggests that Ldb1-/- ES cells have an innate potential to differentiate into neuronal cells, but this potential can be inhibited by environmental influences. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AlphaMed Press Inc | - |
dc.title | The neuronal differentiation potential of Ldb1-null mutant embryonic stem cells is dependent on extrinsic influences | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1634/stemcells.2007-1099 | - |
dc.identifier.scopusid | 2-s2.0-48649100970 | - |
dc.identifier.wosid | 000256907600012 | - |
dc.identifier.bibliographicCitation | Stem Cells, v.26, no.6, pp 1490 - 1495 | - |
dc.citation.title | Stem Cells | - |
dc.citation.volume | 26 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1490 | - |
dc.citation.endPage | 1495 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.url | https://stemcellsjournals.onlinelibrary.wiley.com/doi/full/10.1634/stemcells.2007-1099 | - |
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