Open stomata 1 exhibits dual serine/threonine and tyrosine kinase activity in regulating abscisic acid signaling
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shang, Yun | - |
dc.contributor.author | Yang, Dami | - |
dc.contributor.author | Ha, Yunmi | - |
dc.contributor.author | Lee, Ju Yeon | - |
dc.contributor.author | Kim, Jin Young | - |
dc.contributor.author | Oh, Man-Ho | - |
dc.contributor.author | Nam, Kyoung Hee | - |
dc.date.accessioned | 2022-04-19T09:07:14Z | - |
dc.date.available | 2022-04-19T09:07:14Z | - |
dc.date.issued | 2021-07 | - |
dc.identifier.issn | 0022-0957 | - |
dc.identifier.issn | 1460-2431 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/146499 | - |
dc.description.abstract | Open Stomata 1 (OST1)/SnRK2.6 is a critical component connecting abscisic acid (ABA) receptor complexes and downstream components, including anion channels and transcription factors. Because OST1 is a serine/threonine kinase, several autophosphorylation sites have been identified, and S175 is known to be critical for its kinase activity. We previously reported that BAK1 interacts with and phosphorylates OST1 to regulate ABA signaling. Here, we mapped additional phosphosites of OST1 generated by autophosphorylation and BAK1-mediated transphosphorylation in Arabidopsis. Many phosphosites serve as both auto- and transphosphorylation sites, especially those clustered in the activation loop region. Phospho-mimetic transgenic plants containing quadruple changes in Y163, S164, S166, and S167 rescued ost1 mutant phenotypes, activating ABA signaling outputs. Moreover, we found that OST1 is an active tyrosine kinase, autophosphorylating the Y182 site. ABA induced tyrosine phosphorylation of Y182 in OST1; this event is catalytically important for OST1 activity in plants. ABA-Insensitive 1 (ABI1) and its homologs ABI2 and HAB1, PP2C serine/threonine phosphatases that are known to dephosphorylate OST1 at S175, function as tyrosine phosphatases acting on the phosphorylated Y182 site. Our results indicate that phosphorylation cycles between OST1 and ABI1, which have dual specificity for tyrosine and serine/threonine, coordinately control ABA signaling in Arabidopsis. | - |
dc.format.extent | 14 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | Open stomata 1 exhibits dual serine/threonine and tyrosine kinase activity in regulating abscisic acid signaling | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1093/jxb/erab225 | - |
dc.identifier.scopusid | 2-s2.0-85112572594 | - |
dc.identifier.wosid | 000703237800017 | - |
dc.identifier.bibliographicCitation | JOURNAL OF EXPERIMENTAL BOTANY, v.72, no.15, pp 5494 - 5507 | - |
dc.citation.title | JOURNAL OF EXPERIMENTAL BOTANY | - |
dc.citation.volume | 72 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 5494 | - |
dc.citation.endPage | 5507 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.subject.keywordPlus | PROTEIN PHOSPHATASES | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | OST1 | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | UPSTREAM | - |
dc.subject.keywordPlus | PATTERN | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordAuthor | ABA signaling | - |
dc.subject.keywordAuthor | ABI1 | - |
dc.subject.keywordAuthor | dual-specificity OST1 | - |
dc.subject.keywordAuthor | phosphorylation cycle | - |
dc.subject.keywordAuthor | phosphosites | - |
dc.identifier.url | https://academic.oup.com/jxb/article/72/15/5494/6280244 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Sookmyung Women's University. Cheongpa-ro 47-gil 100 (Cheongpa-dong 2ga), Yongsan-gu, Seoul, 04310, Korea02-710-9127
Copyright©Sookmyung Women's University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.