Enhancing dendritic cell vaccine potency by combining a BAK/BAX siRNA-mediated antiapoptotic strategy to prolong dendritic cell life with an intracellular strategy to target antigen to lysosomal compartments
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Tae Heung | - |
dc.contributor.author | Lee, Jin Hyup | - |
dc.contributor.author | Noh, Kyung Hee | - |
dc.contributor.author | Han, Hee Dong | - |
dc.contributor.author | Shin, Byung Cheol | - |
dc.contributor.author | Choi, Eun Young | - |
dc.contributor.author | Peng, Shiwen | - |
dc.contributor.author | Hung, Chien-Fu | - |
dc.contributor.author | Wu, T. -C. | - |
dc.contributor.author | Kim, Tae Woo | - |
dc.date.available | 2021-02-22T15:02:50Z | - |
dc.date.issued | 2007-04 | - |
dc.identifier.issn | 0020-7136 | - |
dc.identifier.issn | 1097-0215 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/14702 | - |
dc.description.abstract | Dendritic cell (DC)-based vaccines have become important in immunotherapeutics as a measure for generating antitumor immune responses. We have previously demonstrated that linkage of the antigen gene to a lysosomal targeting signal, a sorting signal of the lysosome-associated membrane protein type 1 (LAMP-1), enhances the potency of DC-based vaccines. DCs have a limited life span, hindering their long-term ability to prime antigen-specific T cells. In this study, we attempted to further improve the potency of a DC vaccine that targets human papilloma virus 16 (HPV16) E7 to a lysosomal compartment (DC-Sig/E7/LAMP-1) by combining a strategy to prolong DC life. We show that small interfering RNA-targeting Bak and Bax proteins can be used to allow transfected DCs to resist being killed by T cells. This is done by downregulating these proapoptotic proteins, which have been known as so-called gate keepers in mitochondria-mediated apoptosis. DCs expressing intact E7 or Sig/E7/LAMP-1 became resistant to attack by CD8(+) T cells after transfection with BAK/BAX siRNA, leading to enhanced E7-specilic T cell activation in vitro and in vivo. More importantly, vaccination with E7-presenting DCs transfected with BAK/BAX siRNA generated a strong therapeutic effect against an E7-expressing tumor in vaccinated mice, compared with DCs transfected with control siRNA. Our data indicate that a combination of strategies to enhance intracellular Ag processing and to prolong DC life may offer a promising strategy for improving DC vaccine potency. (c) 2007 Wiley-Liss, Inc. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Enhancing dendritic cell vaccine potency by combining a BAK/BAX siRNA-mediated antiapoptotic strategy to prolong dendritic cell life with an intracellular strategy to target antigen to lysosomal compartments | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1002/ijc.22377 | - |
dc.identifier.scopusid | 2-s2.0-33947148205 | - |
dc.identifier.wosid | 000244700200014 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF CANCER, v.120, no.8, pp 1696 - 1703 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF CANCER | - |
dc.citation.volume | 120 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1696 | - |
dc.citation.endPage | 1703 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | MITOCHONDRIAL-MEMBRANE PERMEABILIZATION | - |
dc.subject.keywordPlus | CLASS-II PRESENTATION | - |
dc.subject.keywordPlus | CANCER-IMMUNOTHERAPY | - |
dc.subject.keywordPlus | ENDOSOMAL/LYSOSOMAL COMPARTMENTS | - |
dc.subject.keywordPlus | ANTITUMOR IMMUNITY | - |
dc.subject.keywordPlus | INTERFERING RNA | - |
dc.subject.keywordPlus | TUMOR-ANTIGEN | - |
dc.subject.keywordPlus | DNA VACCINES | - |
dc.subject.keywordPlus | T-CELLS | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordAuthor | dendritic cell | - |
dc.subject.keywordAuthor | immunotherapy | - |
dc.subject.keywordAuthor | siRNA | - |
dc.subject.keywordAuthor | Sig/E7/LAMP-1 | - |
dc.subject.keywordAuthor | BAK | - |
dc.subject.keywordAuthor | BAX | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Sookmyung Women's University. Cheongpa-ro 47-gil 100 (Cheongpa-dong 2ga), Yongsan-gu, Seoul, 04310, Korea02-710-9127
Copyright©Sookmyung Women's University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.