Chunghyul-dan acts as an anti-inflammatory agent in endothelial cells by regulating gene expression
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정우상 | - |
dc.contributor.author | 조진구 | - |
dc.contributor.author | 인경민 | - |
dc.contributor.author | 김종민 | - |
dc.contributor.author | 조기호 | - |
dc.contributor.author | 박정미 | - |
dc.contributor.author | 문상관 | - |
dc.contributor.author | 김경욱 | - |
dc.contributor.author | 박성욱 | - |
dc.contributor.author | 피재호 | - |
dc.contributor.author | 박상규 | - |
dc.contributor.author | 정윤화 | - |
dc.contributor.author | 박헌용 | - |
dc.contributor.author | 고창남 | - |
dc.date.accessioned | 2022-04-19T10:42:44Z | - |
dc.date.available | 2022-04-19T10:42:44Z | - |
dc.date.issued | 2010-12 | - |
dc.identifier.issn | 1976-8354 | - |
dc.identifier.issn | 2151-2485 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/147847 | - |
dc.description.abstract | Chunghyul-dan (CHD) is a combinatorial drug known to exert anti-inflammatory effects in endothelial cells. In this study, we employed global transcriptional profiling using cDNA microarrays to identify molecular mechanisms responsible for the anti-inflammatory activity of CHD in endothelial cells. An analysis of the microarray data revealed that transcript levels of monocyte chemotactic protein-1 (MCP-1), vascular cell-adhesion molecule-1 (VCAM-1) and activated leukocyte cell-adhesion molecule were dramatically altered in CHD-treated endothelial cells. These changes in gene expression were confirmed by RT-PCR, Western blotting and ELISA. Chronic CHD treatment also appeared to decrease MCP-1 secretion, probably as a result of decreased MCP-1 expression. In addition, we determined that chronic CHD treatment inhibited lipopolysaccharide-stimulated adhesion of THP-1 leukocytes to endothelial cells. The inhibitory effect of CHD on LPS-stimulated adhesion resulted from down-regulation of VCAM-1 expression. Transmigration of THP-1 leukocytes through endothelial cells was also inhibited by chronic CHD treatment. In conclusion, CHD controls a variety of inflammatory activities by regulating MCP-1 and VCAM-1 gene expression. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 한국통합생물학회 | - |
dc.title | Chunghyul-dan acts as an anti-inflammatory agent in endothelial cells by regulating gene expression | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.1080/19768354.2010.525824 | - |
dc.identifier.scopusid | 2-s2.0-79957643697 | - |
dc.identifier.wosid | 000285195800006 | - |
dc.identifier.bibliographicCitation | Animal Cells and Systems, v.14, no.4, pp 275 - 282 | - |
dc.citation.title | Animal Cells and Systems | - |
dc.citation.volume | 14 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 275 | - |
dc.citation.endPage | 282 | - |
dc.identifier.kciid | ART001502603 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.identifier.url | https://www.tandfonline.com/doi/full/10.1080/19768354.2010.525824 | - |
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