p85 β-PIX is required for cell motility through phosphorylations of focal adhesion kinase and p38 MAP kinase
DC Field | Value | Language |
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dc.contributor.author | Jangsoon Lee | - |
dc.contributor.author | In Duk Jung | - |
dc.contributor.author | Won Keun Chang | - |
dc.contributor.author | Do Yeun Cho | - |
dc.contributor.author | Eun-Young Shin | - |
dc.contributor.author | Dong Wan Seo | - |
dc.contributor.author | 김용기 | - |
dc.contributor.author | Hyang Woo Lee | - |
dc.contributor.author | Jeung-Whan Han | - |
dc.contributor.author | Hoi Young Lee | - |
dc.date.accessioned | 2022-04-19T11:42:40Z | - |
dc.date.available | 2022-04-19T11:42:40Z | - |
dc.date.issued | 2005-07 | - |
dc.identifier.issn | 0014-4827 | - |
dc.identifier.issn | 1090-2422 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/148759 | - |
dc.description.abstract | Lysophosphatidic acid (LPA) mediates diverse biological responses, including cell migration, through the activation of G-protein-coupled receptors. Recently, we have shown that LPA stimulates p21-activated kinase (PAK) that is critical for focal adhesion kinase (FAK) phosphorylation and cell motility. Here, we provide the direct evidence that p85 β-PIX is required for cell motility of NIH-3T3 cells by LPA through FAK and p38 MAP kinase phosphorylations. LPA induced p85 β-PIX binding to FAK in NIH-3T3 cells that was inhibited by pretreatment of the cells with phosphoinositide 3-kinase inhibitor, LY294002. Furthermore, the similar inhibition of the complex formation was also observed, when the cells were transfected with either p85 β-PIX mutant that cannot bind GIT or dominant negative mutants of Rac1 (N17Rac1) and PAK (PAK-PID). Transfection of the cells with specific p85 β-PIX siRNA led to drastic inhibition of LPA-induced FAK phosphorylation, peripheral redistribution of p85 β-PIX with FAK and GIT1, and cell motility. p85 β-PIX was also required for p38 MAP kinase phosphorylation induced by LPA. Finally, dominant negative mutant of Rho (N19Rho)-transfected cells did not affect PAK activation, while the cells stably transfected with p85 β-PIX siRNA or N17Rac1 showed the reduction of LPA-induced PAK activation. Taken together, the present data suggest that p85 β-PIX, located downstream of Rac1, is a key regulator for the activations of FAK or p38 MAP kinase and plays a pivotal role in focal complex formation and cell motility induced by LPA. © 2005 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 14 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Academic Press | - |
dc.title | p85 β-PIX is required for cell motility through phosphorylations of focal adhesion kinase and p38 MAP kinase | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.yexcr.2005.03.028 | - |
dc.identifier.scopusid | 2-s2.0-20444374515 | - |
dc.identifier.wosid | 000230068800004 | - |
dc.identifier.bibliographicCitation | Experimental Cell Research, v.307, no.2, pp 315 - 328 | - |
dc.citation.title | Experimental Cell Research | - |
dc.citation.volume | 307 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 315 | - |
dc.citation.endPage | 328 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0014482705001059?pes=vor | - |
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