Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme for protein ISGylation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim KI | - |
dc.contributor.author | Giannakopoulos NV | - |
dc.contributor.author | Virgin HW | - |
dc.contributor.author | Zhang DE | - |
dc.date.accessioned | 2022-04-19T11:44:50Z | - |
dc.date.available | 2022-04-19T11:44:50Z | - |
dc.date.issued | 2004-11 | - |
dc.identifier.issn | 0270-7306 | - |
dc.identifier.issn | 1098-5549 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/148880 | - |
dc.description.abstract | Protein ISGylation is unique among ubiquitin-like conjugation systems in that the expression and conjugation processes are induced by specific stimuli, mainly via the alpha/beta interferon signaling pathway. It has been suggested that protein ISGylation plays a special role in the immune response, because of its interferonsignal dependency and its appearance only in higher eukaryotic organisms. Here, we report the identification of an ISG15-conjugating enzyme, Ubc8. Like other components of the protein ISGylation system (ISG15, UBE1L, and UBP43), Ubc8 is an interferon-inducible protein. Ubc8 clearly mediates protein ISGylation in transfection assays. The reduction of Ubc8 expression by small interfering RNA causes a decrease in protein ISGylation in HeLa cells upon interferon treatment. Neither UbcH7/UbcM4, the closest homologue of Ubc8 among known ubiquitin E2s, nor the small ubiquitin-like modifier E2 Ubc9 supports protein ISGylation. These findings strongly suggest that Ubc8 is a major ISG15-conjugating enzyme responsible for protein ISGylation upon interferon stimulation. Furthermore, we established an assay system to detect ISGylated target proteins by cotransfection of ISG15, UBE1L, and Ubc8 together with a target protein to be analyzed. This method provides an easy and effective way to identify new target | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme for protein ISGylation | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1128/MCB.24.21.9592-9600.2004 | - |
dc.identifier.scopusid | 2-s2.0-6344282196 | - |
dc.identifier.wosid | 000224943300030 | - |
dc.identifier.bibliographicCitation | MOLECULAR AND CELLULAR BIOLOGY, v.24, no.21, pp 9592 - 9600 | - |
dc.citation.title | MOLECULAR AND CELLULAR BIOLOGY | - |
dc.citation.volume | 24 | - |
dc.citation.number | 21 | - |
dc.citation.startPage | 9592 | - |
dc.citation.endPage | 9600 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.url | https://journals.asm.org/doi/10.1128/MCB.24.21.9592-9600.2004 | - |
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