Protein patterning by maskless photolithography on hydrophilic polymer-grafted surface
DC Field | Value | Language |
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dc.contributor.author | Shin, DS | - |
dc.contributor.author | Lee, KN | - |
dc.contributor.author | Janga, KH | - |
dc.contributor.author | Kim, JK | - |
dc.contributor.author | Chung, WJ | - |
dc.contributor.author | Kim, YK | - |
dc.contributor.author | Lee, YS | - |
dc.date.accessioned | 2022-04-19T12:02:26Z | - |
dc.date.available | 2022-04-19T12:02:26Z | - |
dc.date.issued | 2003-12 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.issn | 1873-4235 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/149046 | - |
dc.description.abstract | With the help of a microfabrication process and surface modification technology, a method of fabricating protein patterned chips was developed which can be utilized as a powerful tool for performing bioassays in a high-throughput manner. A digital micromirror array (MMA) system was used as a virtual photomask, so that a maskless photolithography process was able to be used to build patterned biomolecules on a chip by selective illumination onto the chip surface. We utilized the nitroveratryloxycarbonyl (NVOC) group as a photolabile protecting group for protein patterning. The NVOC-protected surface was selectively irradiated by a UV illuminator using an MMA. After removing the NVOC group, biotin was coupled to the NVOC-cleaved site, onto which a buffered streptavidin solution was eluted. At this point, we could obtain a streptavidin-patterned surface and observe the effect of the polymer-grafted surface in reducing nonspecific binding. (C) 2003 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Protein patterning by maskless photolithography on hydrophilic polymer-grafted surface | - |
dc.type | Article | - |
dc.publisher.location | 네델란드 | - |
dc.identifier.doi | 10.1016/S0956-5663(03)00228-8 | - |
dc.identifier.scopusid | 2-s2.0-0242667526 | - |
dc.identifier.wosid | 000187011000010 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.19, no.5, pp 485 - 494 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 19 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 485 | - |
dc.citation.endPage | 494 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Maskless photolithography | - |
dc.subject.keywordAuthor | Micromirror array | - |
dc.subject.keywordAuthor | Nonspecific binding | - |
dc.subject.keywordAuthor | Polymer-grafted surface | - |
dc.subject.keywordAuthor | Protein chip | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0956566303002288?via%3Dihub | - |
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