Dexamethasone reverses TGF-beta-mediated inhibition of primary rat preadipocyte differentiation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, SM | - |
dc.contributor.author | Kim, KY | - |
dc.contributor.author | Kim, JK | - |
dc.contributor.author | Yoon, SR | - |
dc.contributor.author | Choi, I | - |
dc.contributor.author | Yang, Y | - |
dc.date.accessioned | 2022-04-19T12:04:37Z | - |
dc.date.available | 2022-04-19T12:04:37Z | - |
dc.date.issued | 2003-05 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.issn | 1873-3468 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/149159 | - |
dc.description.abstract | Dexamethasone and transforming growth factor-beta (TGF-beta) show contrary effects on differentiation of adipocytes. Dexamethasone stimulates adipocyte differentiation whereas TGF-beta inhibits it. In the present study, we investigated whether dexamethasone could reverse the TGF-beta-mediated inhibition of preadipocyte differentiation. Primary rat preadipocytes, obtained from Sprague-Dawley rats, were pretreated with dexamethasone in the presence or absence of TGF-beta, prior to the induction of differentiation. Co-treatment of dexamethasone and TGF-beta before inducing differentiation reversed the TGF-beta-mediated inhibition of preadipocyte differentiation. In order to elucidate the mechanism by which dexamethasone reversed the effect of TGF-beta on the inhibition of preadipocyte differentiation, the expression of CCAAT/enhancer binding protein-alpha (C/EBPalpha) and peroxisome proliferator-activated receptorgamma(PPARgamma) was examined. Dexamethasone increased C/EBPalpha and PPARgamma expression in the absence of TGF-beta and also recovered the TGF-beta-mediated suppression of C/EBPalpha expression in preadipocytes. Its effect was sustained in differentiated adipocytes as well. However, those effects were not observed in 3T3-L1 preadipocytes or differentiated adipocytes. These results indicate that dexametha | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Dexamethasone reverses TGF-beta-mediated inhibition of primary rat preadipocyte differentiation | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/S0014-5793(03)00371-5 | - |
dc.identifier.scopusid | 2-s2.0-0037716992 | - |
dc.identifier.wosid | 000183121500006 | - |
dc.identifier.bibliographicCitation | FEBS LETTERS, v.543, no.1-3, pp 25 - 30 | - |
dc.citation.title | FEBS LETTERS | - |
dc.citation.volume | 543 | - |
dc.citation.number | 1-3 | - |
dc.citation.startPage | 25 | - |
dc.citation.endPage | 30 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | CCAAT/enhancer binding protein-α | - |
dc.subject.keywordAuthor | Dexamethasone | - |
dc.subject.keywordAuthor | Peroxisome proliferator-activated receptor γ | - |
dc.subject.keywordAuthor | Rat preadipocyte | - |
dc.subject.keywordAuthor | Transforming growth factor-β | - |
dc.identifier.url | https://febs.onlinelibrary.wiley.com/doi/full/10.1016/S0014-5793%2803%2900371-5 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Sookmyung Women's University. Cheongpa-ro 47-gil 100 (Cheongpa-dong 2ga), Yongsan-gu, Seoul, 04310, Korea02-710-9127
Copyright©Sookmyung Women's University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.