Ergolide, sesquiterpene lactone from Inula britannica, inhibits inducible nitric oxide synthase and cyclo-oxygenase-2 expression in RAW 264.7 macrophages through the inactivation of NF-kappaB
DC Field | Value | Language |
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dc.contributor.author | Jeung Whan Han | - |
dc.contributor.author | Byeong Gon Lee | - |
dc.contributor.author | 김용기 | - |
dc.contributor.author | Jong Woo Yoon | - |
dc.contributor.author | Hye Kyoung Jin | - |
dc.contributor.author | Sungyoul Hong | - |
dc.contributor.author | Hoi Young Lee | - |
dc.contributor.author | Kang Ro Lee | - |
dc.contributor.author | Hyang Woo Lee | - |
dc.date.accessioned | 2022-04-19T12:25:47Z | - |
dc.date.available | 2022-04-19T12:25:47Z | - |
dc.date.issued | 2001-06 | - |
dc.identifier.issn | 0007-1188 | - |
dc.identifier.issn | 1476-5381 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/149586 | - |
dc.description.abstract | 1 We investigated the mechanism of suppression of inducible nitric oxide synthase (iNOS) and cyclo-oxygenase-2 (COX-2) by ergolide, sesquiterpene lactone from Inula britannica. 2 iNOS activity in cell-free extract of LPS/IFN-gamma -stimulated RAW 264.7 macrophages was markedly attenuated by the treatment with ergolide. Its inhibitory effect on iNOS was paralleled by decrease in nitrite accumulation in culture medium of LPS/IFN-gamma -stimulated RAW 264.7 macrophages in a concentration-dependent manner. However, its inhibitory effect does not result from direct inhibition of the catalytic activity of NOS. 3 Ergolide markedly decreased the production of prostaglandin E-2 (PGE(2)) in cell-free extract of LPS/IFN-gamma -stimulated RAW 264.7 macrophages in a concentration-dependent manner, without alteration of the catalytic activity of COX-2 itself. 4 Ergolide decreased the level of iNOS and COX-2 protein, and iNOS mRNA caused by stimulation of LPS/IFN-gamma in a concentration-dependent manner, as measured by Western blot and Northern blot analysis, respectively. 5 Ergolide inhibited nuclear factor-kappaB (NF-kappaB) activation, a transcription factor necessary for iNOS and COX-2 expression in response to LPS/IFN-gamma. This effect was accompanied by the parallel reduction of nuclear translocation of subunit p65 of NF-kappaB as well as I kappaB-alpha degradation. In addition, these effects were completely blocked by treatment of cysteine, indicating that this inhibitory effect of ergolide could be mediated by alkylation of NF-kappaB itself or an upstream molecule of NF-kappaB. 6 Ergolide also directly inhibited the DNA-binding activity of active NF-kappaB in LPS/IFN-gamma- pretreated RAW 264.7 macrophages. 7 These results demonstrate that the suppression of NF-kappaB activation by ergolide might be attributed to the inhibition of nuclear translocation of NF-kappaB resulted from blockade of the degradation of I kappaB and the direct modification of active NF-kappaB, leading to the suppression of the expression of iNOS and COX-2, which play important roles in inflammatory signalling pathway. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Ergolide, sesquiterpene lactone from Inula britannica, inhibits inducible nitric oxide synthase and cyclo-oxygenase-2 expression in RAW 264.7 macrophages through the inactivation of NF-kappaB | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1038/sj.bjp.0704099 | - |
dc.identifier.scopusid | 2-s2.0-0034955767 | - |
dc.identifier.wosid | 000169433900007 | - |
dc.identifier.bibliographicCitation | Br J Pharmacol, v.133, no.4, pp 503 - 512 | - |
dc.citation.title | Br J Pharmacol | - |
dc.citation.volume | 133 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 503 | - |
dc.citation.endPage | 512 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | RAW-264.7 MACROPHAGES | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | SUPPRESSION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | PROSTAGLANDINS | - |
dc.subject.keywordAuthor | ergolide | - |
dc.subject.keywordAuthor | inducible nitric oxide synthase | - |
dc.subject.keywordAuthor | cyclo-oxygenase-2 | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | I kappa B | - |
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