Intrinsic transcriptional activation-inhibition domains of the polyomavirus enhancer binding protein 2 core binding factor alpha subunit revealed in the presence of the beta subunit

Abstract

A member of the polyomavirus enhancer binding protein 2/core binding factor (PEBP2/CBF) is composed of PEBP2 alpha B1/AML1 (as the alpha subunit) and a beta subunit. It plays an essential role in definitive hematopoiesis and is frequently involved in the chromosomal abnormalities associated with leukemia. In the present study, we report functionally separable modular structures in PEBP2 alpha B1 for DNA binding and for transcriptional activation. DNA binding through the Runt domain of PEBP2 alpha B1 was hindered by the adjacent carboxyterminal region, and this inhibition was relieved by interaction with the beta subunit. Utilizing a reporter assay system in which both the alpha and beta subunits are required to achieve strong transactivation, we uncovered the presence of transcriptional activation and inhibitory domains in PEBP2 alpha B1 that were only apparent in the presence of the beta subunit. The inhibitory domain keeps the full transactivation potential of full-length PEBP2 alpha B1 below its maximum potential. Fusion of the transactivation domain of PEBP2 alpha B1 to the yeast GAL4 DNA-binding domain conferred transactivation potential, but further addition of the inhibitory domain diminished the activity. These results suggest that the activity of the alpha subunit as a transcriptional activator is regul