Tn5 Tagging of the Phenol-degrading Gene on the Chromosome of Pseudomonas putida
DC Field | Value | Language |
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dc.contributor.author | Han E.-M. | - |
dc.contributor.author | Jung Y.-H. | - |
dc.contributor.author | On H.-Y. | - |
dc.contributor.author | Lee M.-S. | - |
dc.contributor.author | Min K.-H. | - |
dc.contributor.author | Yang Y.-K. | - |
dc.contributor.author | Kim Y. | - |
dc.contributor.author | Kim C.-K. | - |
dc.contributor.author | Lee K.-S. | - |
dc.date.accessioned | 2022-04-19T13:41:56Z | - |
dc.date.available | 2022-04-19T13:41:56Z | - |
dc.date.issued | 1997-02 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.issn | 0219-1032 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/150525 | - |
dc.description.abstract | Transposon mutagenesis was performed by the method of conjugational transfer in order to identify and characterize genes encoding enzymes involved in the pathway of phenol utilization as a carbon source. Escherichia coli, which carries the Tn5-132, Was mated with Pseudomonas putida SM25 as a host. We selected a mutant that could not utilize phenol as a carbon source. Chromosomal integration of the transposon was confirmed by Southern analysis, successfully tagging the gene related to a phenol-utilizing pathway. By cell-free enzyme and genetic complementation assays, the inactivated enzyme through the mutation of the corresponding gene was identified as the catB gene, which encodes a cis,cis-muconate lactonizing enzyme. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Springer Verlag | - |
dc.title | Tn5 Tagging of the Phenol-degrading Gene on the Chromosome of Pseudomonas putida | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.scopusid | 2-s2.0-0031588619 | - |
dc.identifier.wosid | A1997WK97900006 | - |
dc.identifier.bibliographicCitation | Molecules and Cells, v.7, no.1, pp 40 - 44 | - |
dc.citation.title | Molecules and Cells | - |
dc.citation.volume | 7 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 40 | - |
dc.citation.endPage | 44 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.url | https://www.molcells.org/journal/view.html?pn=vol&uid=2864&vmd=Full#body00 | - |
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