Selection of peptides that bind to the HLA-A2.1 molecule by molecular modelling
DC Field | Value | Language |
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dc.contributor.author | Lim, JS | - |
dc.contributor.author | Kim, S | - |
dc.contributor.author | Lee, HG | - |
dc.contributor.author | Lee, KY | - |
dc.contributor.author | Kwon, TJ | - |
dc.contributor.author | Kim, K | - |
dc.date.accessioned | 2022-04-19T13:45:53Z | - |
dc.date.available | 2022-04-19T13:45:53Z | - |
dc.date.issued | 1996-02 | - |
dc.identifier.issn | 0161-5890 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/150772 | - |
dc.description.abstract | Cytotoxic T lymphocytes recognize antigenic peptides in association with major histocompatibility complex class I proteins. Although a large set of class I binding peptides has been described, it is not yet easy to search for potentially antigenic peptides without synthesis of a panel of peptides, and subsequent binding assays. In order to predict HLA-A2.1-restricted antigenic epitopes, a computer model of the HLA-A2.1 molecule was established using X-ray crystallography data. In this model nonameric peptide sequences were aligned. In a molecular dynamics (MD) simulation with two sets of peptides known to be presented by HLA-A2.1, it was important to know the anchor amino acid residue preference and the distance between the anchor residues. We show here that the peptides bound to the HLA-A2.1 model structure possess a side chain of C-terminal anchor residue oriented into the binding groove with different distances between the two anchor residues from 15 to 21 Angstrom. We also synthesized a set of nonamer peptides containing amino acid sequences of Hepatitis B virus protein that were selected on the basis of previously described HLA-A2.1 specific motifs. When results obtained from the MD simulation were compared with functional binding assays using the TAP-deficient cell line T2, it was evident that the MD simul | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | Selection of peptides that bind to the HLA-A2.1 molecule by molecular modelling | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1016/0161-5890(95)00065-8 | - |
dc.identifier.scopusid | 2-s2.0-0029926327 | - |
dc.identifier.wosid | A1996UH90400010 | - |
dc.identifier.bibliographicCitation | MOLECULAR IMMUNOLOGY, v.33, no.2, pp 221 - 230 | - |
dc.citation.title | MOLECULAR IMMUNOLOGY | - |
dc.citation.volume | 33 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 221 | - |
dc.citation.endPage | 230 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | computer modeling | - |
dc.subject.keywordAuthor | epitope prediction | - |
dc.subject.keywordAuthor | MHC binding peptide | - |
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