Effects of heterologous expression of thioredoxin reductase on the level of reactive oxygen species in COS-7 cells
DC Field | Value | Language |
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dc.contributor.author | Kang, Hyun-Jung | - |
dc.contributor.author | Hong, Sung-Min | - |
dc.contributor.author | Kim, Byung-Chul | - |
dc.contributor.author | Park, Eun-Hee | - |
dc.contributor.author | Ahn, Kisup | - |
dc.contributor.author | Lim, Chang-Jin | - |
dc.date.available | 2021-02-22T15:18:15Z | - |
dc.date.issued | 2006-08 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.issn | 0219-1032 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15078 | - |
dc.description.abstract | Thioredoxin reductase (TrxR), a component of the redox control system involving thioredoxin (Trx), is implicated in defense against oxidative stress, control of cell growth and proliferation, and regulation of apoptosis. In the present study a stable transfectant was made by introducing the vector pcDNA3.0 harboring the fission yeast TrxR gene into COS-7 African green monkey kidney fibroblast cells. The exogenous TrxR gene led to an increase in TrxR activity of up to 3.2-fold but did not affect glutathione (GSH) content, or glutaredoxin and caspase-3 activities. Levels of reactive oxygen species (ROS), but not those of nitric oxide (NO), were reduced. Conversely, 1-chloro-2,4-dinitrobezene (CDNB), an irreversible inhibitor of mammalian TrxR, enhanced ROS levels in the COS-7 cells. After treatment with hydrogen peroxide, the level of intracellular ROS was lower in the transfectants than in the vector control cells. These results confirm that TrxR is a crucial determinant of the level of cellular ROS during oxidative stress as well as in the normal state. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.title | Effects of heterologous expression of thioredoxin reductase on the level of reactive oxygen species in COS-7 cells | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.wosid | 000240323200016 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.22, no.1, pp 113 - 118 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 22 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 113 | - |
dc.citation.endPage | 118 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001022169 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | VEIN ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | LINES | - |
dc.subject.keywordPlus | THIOLTRANSFERASE | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordAuthor | COS-7 | - |
dc.subject.keywordAuthor | hydrogen peroxide | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordAuthor | thioredoxin reductase | - |
dc.identifier.url | https://www.molcells.org/journal/view.html?year=2006&volume=22&number=1&spage=113 | - |
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