Structural and Biochemical Insights into Bis(2-hydroxyethyl) Terephthalate Degrading Carboxylesterase Isolated from Psychrotrophic Bacterium Exiguobacterium antarcticum
DC Field | Value | Language |
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dc.contributor.author | Hwang, Jisub | - |
dc.contributor.author | Yoo, Wanki | - |
dc.contributor.author | Shin, Seung Chul | - |
dc.contributor.author | Kim, Kyeong Kyu | - |
dc.contributor.author | Kim, Han-Woo | - |
dc.contributor.author | Do, Hackwon | - |
dc.contributor.author | Lee, Jun Hyuck | - |
dc.date.accessioned | 2023-11-08T05:47:15Z | - |
dc.date.available | 2023-11-08T05:47:15Z | - |
dc.date.issued | 2023-08 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/151684 | - |
dc.description.abstract | This study aimed to elucidate the crystal structure and biochemically characterize the carboxylesterase EaEst2, a thermotolerant biocatalyst derived from Exiguobacterium antarcticum, a psychrotrophic bacterium. Sequence and phylogenetic analyses showed that EaEst2 belongs to the Family XIII group of carboxylesterases. EaEst2 has a broad range of substrate specificities for short-chain p-nitrophenyl (pNP) esters, 1-naphthyl acetate (1-NA), and 1-naphthyl butyrate (1-NB). Its optimal pH is 7.0, losing its enzymatic activity at temperatures above 50 & DEG;C. EaEst2 showed degradation activity toward bis(2-hydroxyethyl) terephthalate (BHET), a polyethylene terephthalate degradation intermediate. We determined the crystal structure of EaEst2 at a 1.74 & ANGS; resolution in the ligand-free form to investigate BHET degradation at a molecular level. Finally, the biochemical stability and immobilization of a crosslinked enzyme aggregate (CLEA) were assessed to examine its potential for industrial application. Overall, the structural and biochemical characterization of EaEst2 demonstrates its industrial potency as a biocatalyst. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI | - |
dc.title | Structural and Biochemical Insights into Bis(2-hydroxyethyl) Terephthalate Degrading Carboxylesterase Isolated from Psychrotrophic Bacterium Exiguobacterium antarcticum | - |
dc.type | Article | - |
dc.publisher.location | 스위스 | - |
dc.identifier.doi | 10.3390/ijms241512022 | - |
dc.identifier.scopusid | 2-s2.0-85167753921 | - |
dc.identifier.wosid | 001046328100001 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.24, no.15 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 24 | - |
dc.citation.number | 15 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | POLYETHYLENE TEREPHTHALATE | - |
dc.subject.keywordPlus | ENZYMATIC DEGRADATION | - |
dc.subject.keywordPlus | POLYESTER HYDROLASE | - |
dc.subject.keywordPlus | REFINEMENT | - |
dc.subject.keywordPlus | ESTERASES | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordAuthor | carboxylesterases | - |
dc.subject.keywordAuthor | Exiguobacterium antarcticum | - |
dc.subject.keywordAuthor | biocatalyst | - |
dc.identifier.url | https://accesson.kisti.re.kr/main/search/articleDetail.do?artiId=ATN0044452975 | - |
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