A bio-friendly biotin-coupled and azide-functionalized naphthalimide for real-time endogenous hydrogen sulfide analysis in living cells
DC Field | Value | Language |
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dc.contributor.author | Cha, Yujin | - |
dc.contributor.author | Gopala, Lavanya | - |
dc.contributor.author | Lee, Min Hee | - |
dc.date.accessioned | 2023-11-08T06:45:24Z | - |
dc.date.available | 2023-11-08T06:45:24Z | - |
dc.date.issued | 2023-04 | - |
dc.identifier.issn | 1386-1425 | - |
dc.identifier.issn | 1873-3557 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/151885 | - |
dc.description.abstract | Hydrogen sulfide (H2S) is involved in various biological processes. Thereby, abnormal levels of H2S are reported to be related to various human diseases including cancer. Currently, many fluorescent probes are pioneered to detect H2S by taking advantages of naphthalimides’ unique internal charge transfer (ICT) property. However, most probes often require a high content of organic solvents or surfactants, and are limited to the analysis of exogenous H2S treated externally in live cell studies, and have difficulties in analyzing endogenous H2S, thus limiting their practical use. In this study, we developed a bio-friendly biotin-coupled and azide-functionalized naphthalimide (1) as a fluorescent probe enabling real-time analysis of H2S in living system. Probe was able to provide a fluorescence at 545 nm via H2S-mediated azide reduction selectively without interference by biologically abundant constituents and pH effects. In a biological study using A549 cells, probe readily penetrated living cells without cytotoxicity, and unreacted probes showed almost no fluorescence, enabling real-time detection of H2S in living cells without requiring separate washing process. More importantly, under stimulation with various H2S inducers and inhibitors, probe was able to provide an effective fluorescence response against fluctuations in endogenous H2S, a key requirement for H2S studies. Probe 1 can be applied as a useful chemical tool and enables the analysis of H2S and the study of H2S-related cell functions in a variety of environments. © 2023 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier B.V. | - |
dc.title | A bio-friendly biotin-coupled and azide-functionalized naphthalimide for real-time endogenous hydrogen sulfide analysis in living cells | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1016/j.saa.2023.122385 | - |
dc.identifier.scopusid | 2-s2.0-85146631540 | - |
dc.identifier.wosid | 000996066500001 | - |
dc.identifier.bibliographicCitation | Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, v.291 | - |
dc.citation.title | Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | - |
dc.citation.volume | 291 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Spectroscopy | - |
dc.relation.journalWebOfScienceCategory | Spectroscopy | - |
dc.subject.keywordPlus | CYSTATHIONINE-BETA-SYNTHASE | - |
dc.subject.keywordPlus | FLUORESCENT-PROBES | - |
dc.subject.keywordPlus | H2S | - |
dc.subject.keywordPlus | HYPERTENSION | - |
dc.subject.keywordAuthor | Biotin | - |
dc.subject.keywordAuthor | Endogenous H2S | - |
dc.subject.keywordAuthor | Fluorescent probe | - |
dc.subject.keywordAuthor | Live cell | - |
dc.subject.keywordAuthor | Naphthalimide | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1386142523000707?via%3Dihub | - |
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