조골세포에 있어서 형질전환 효율의 최적화

Abstract

Achievement of optimal transfection efficiency is invaluable for mechanistic studies on osteoblastic gene regulation. In the present study, we have shown that the efficiency of biochemical transfection reagents varies significantly in UAMS-32 osteoblastic cells. The highest transfection efficiency was obtained when 4 mg DNA and Welfect-plus ™ was used in UAMS-32 osteoblastic cells. We also showed the evidence of the applicability of this method for the efficient transfeption of osteoblastic cells with reporter constructs for luciferase reporter assay.