Electrochemical Impedance-Based Biosensors for the Label-Free Detection of the Nucleocapsid Protein from SARS-CoV-2
DC Field | Value | Language |
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dc.contributor.author | Cho, Hana | - |
dc.contributor.author | Shim, Suhyun | - |
dc.contributor.author | Cho, Won Woo | - |
dc.contributor.author | Cho, Sungbo | - |
dc.contributor.author | Baek, Hanseung | - |
dc.contributor.author | Lee, Sang-Myung | - |
dc.contributor.author | Shin, Dong-Sik | - |
dc.date.accessioned | 2023-11-08T09:44:02Z | - |
dc.date.available | 2023-11-08T09:44:02Z | - |
dc.date.issued | 2022-06 | - |
dc.identifier.issn | 2379-3694 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/152728 | - |
dc.description.abstract | Diagnosis of coronavirus disease (COVID-19) is important because of the emergence and global spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Real-time polymerase chain reaction (PCR) is widely used to diagnose COVID-19, but it is time-consuming and requires sending samples to test centers. Thus, the need to detect antigens for rapid on-site diagnosis rather than PCR is increasing. We quantified the nucleocapsid (N) protein in SARS-CoV-2 using an electro-immunosorbent assay (El-ISA) and a multichannel impedance analyzer with a 96-interdigitated microelectrode sensor (ToAD). The El-ISA measures impedance signals from residual detection antibodies after sandwich assays and thus offers highly specific, label-free detection of the N protein with low cross-reactivity. The ToAD sensor enables the real-time electrochemical detection of multiple samples in conventional 96-well plates. The limit of detection for the N protein was 0.1 ng/mL with a detection range up to 10 ng/mL. This system did not detect signals for the S protein. While this study focused on detecting the N protein in SARS-CoV-2, our system can also be widely applicable to detecting various biomolecules involved in antigen-antibody interactions. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Electrochemical Impedance-Based Biosensors for the Label-Free Detection of the Nucleocapsid Protein from SARS-CoV-2 | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1021/acssensors.2c00317 | - |
dc.identifier.scopusid | 2-s2.0-85132128957 | - |
dc.identifier.wosid | 000818560300001 | - |
dc.identifier.bibliographicCitation | ACS SENSORS, v.7, no.6, pp 1676 - 1684 | - |
dc.citation.title | ACS SENSORS | - |
dc.citation.volume | 7 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1676 | - |
dc.citation.endPage | 1684 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | FLOW IMMUNO ASSAY | - |
dc.subject.keywordPlus | FLUORESCENCE | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordAuthor | electrochemical sensor | - |
dc.subject.keywordAuthor | nucleocapsid protein | - |
dc.subject.keywordAuthor | SARS-CoV-2 | - |
dc.subject.keywordAuthor | impedance | - |
dc.subject.keywordAuthor | label-free detection | - |
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