The fission yeast gene encoding monothiol glutaredoxin 5 is regulated by nitrosative and osmotic stresses
DC Field | Value | Language |
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dc.contributor.author | Kim, HG | - |
dc.contributor.author | Park, EH | - |
dc.contributor.author | Lim, CJ | - |
dc.date.available | 2021-02-22T15:46:30Z | - |
dc.date.issued | 2005-08 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.issn | 0219-1032 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15482 | - |
dc.description.abstract | Glutaredoxin (Grx) is a small, heat-stable redox protein acting as a multi-functional glutathione (GSH)-dependent disulfide oxidoreductase. We have cloned the monothiol Grx5 gene from the genomic DNA of the fission yeast Schizosaccharomyces pombe. It has 1,904 bp, with one intron, and encodes a putative protein of 146 amino acids with a molecular mass of 16.5 kDa. Recombinant Grx5 produced functional Grx in S. pombe cells. NO-generating sodium nitroprusside (SNP, 1.0 and 2.0 mM) and potassium chloride (KCl, 0.2 and 0.5 M) increased the synthesis of beta-galactosidase from a Grx5-lacZ fusion gene, and transcription of Grx5 was also enhanced by SNP and KCl. Synthesis of beta-galactosidase from the Grx5-lacZ fusion was lower in Pap1-negative TP108-3C cells than in wild type KP1 cells, and when PapI was overproduced in KP1 cells, the level of P-galactosidase increased. We also found that PapI is involved in the induction of Grx5 by SNP and KCl. S. pombe Grx5 may play a crucial role in responses to nitrosative and osmotic stresses. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.title | The fission yeast gene encoding monothiol glutaredoxin 5 is regulated by nitrosative and osmotic stresses | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.scopusid | 2-s2.0-33644698717 | - |
dc.identifier.wosid | 000231701500005 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.20, no.1, pp 43 - 50 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 20 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 43 | - |
dc.citation.endPage | 50 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001095935 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | METABOLIC OXIDATIVE STRESS | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | THIOLTRANSFERASE GLUTAREDOXIN | - |
dc.subject.keywordPlus | SCHIZOSACCHAROMYCES-POMBE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | PROTEIN-KINASE | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | LACZ FUSIONS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | THIOREDOXIN | - |
dc.subject.keywordAuthor | Grx5-lacZ | - |
dc.subject.keywordAuthor | monothiol glutaredoxin | - |
dc.subject.keywordAuthor | Pap1 | - |
dc.subject.keywordAuthor | regulation | - |
dc.subject.keywordAuthor | S. pombe | - |
dc.subject.keywordAuthor | stress response | - |
dc.identifier.url | https://www.molcells.org/journal/view.html?year=2005&volume=20&number=1&spage=43 | - |
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